TY - JOUR

T1 - Histopathology in the Diagnosis of Invasive Fungal Diseases

AU - Jensen, Henrik Elvang

N1 - Publisher Copyright: © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.

PY - 2021

Y1 - 2021

N2 - Purpose of Review: The classical diagnostic principles for applying histopathology for the diagnosis of invasive fungal diseases are reviewed. Although several new molecular based techniques have recently been developed, the histopathological identification of fungal elements together with a typical tissue reaction remains the golden standard for stating a diagnosis of invasive mycosis. Therefore, and due to the risk of false negative and false positive results obtained from cultivation as well as the non-culture based diagnostic test for invasive fungal infections, an examination should always complement histopathology in the diagnosis of invasive fungal diseases. Recent Findings: The application of molecular in situ identification techniques, i.e., immunohistochemistry and in situ hybridization, for morphologically observed fungal elements in tissue sections, has indeed improved the diagnostic accuracy of histopathology for the diagnosis of invasive fungal diseases. Summary: Because the specific molecular techniques applied in the histopathological diagnosis of invasive mycoses are directed toward specific targets, the panel of specific immunoglobulins/probes to be used on tissue sections should be directed from the histomorphology of the fungal elements as detected by conventional histopathological methods.

AB - Purpose of Review: The classical diagnostic principles for applying histopathology for the diagnosis of invasive fungal diseases are reviewed. Although several new molecular based techniques have recently been developed, the histopathological identification of fungal elements together with a typical tissue reaction remains the golden standard for stating a diagnosis of invasive mycosis. Therefore, and due to the risk of false negative and false positive results obtained from cultivation as well as the non-culture based diagnostic test for invasive fungal infections, an examination should always complement histopathology in the diagnosis of invasive fungal diseases. Recent Findings: The application of molecular in situ identification techniques, i.e., immunohistochemistry and in situ hybridization, for morphologically observed fungal elements in tissue sections, has indeed improved the diagnostic accuracy of histopathology for the diagnosis of invasive fungal diseases. Summary: Because the specific molecular techniques applied in the histopathological diagnosis of invasive mycoses are directed toward specific targets, the panel of specific immunoglobulins/probes to be used on tissue sections should be directed from the histomorphology of the fungal elements as detected by conventional histopathological methods.

KW - Fungi

KW - Histopathology

KW - Immunohistochemistry

KW - In situ hybridization

KW - Mycoses

KW - Tissue

U2 - 10.1007/s12281-021-00412-y

DO - 10.1007/s12281-021-00412-y

M3 - Review

AN - SCOPUS:85100877911

VL - 15

SP - 23

EP - 31

JO - Current Fungal Infection Reports

JF - Current Fungal Infection Reports

SN - 1936-3761

IS - 1

ER -