“Non-healing” claw horn lesions in dairy cows: Clinical, histopathological and molecular biological characterization of four cases
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
“Non-healing” claw horn lesions in dairy cows : Clinical, histopathological and molecular biological characterization of four cases. / Alsaaod, Maher; Weber, Jim; Jensen, Tim; Brandt, Sabine; Gurtner, Corinne; Devaux, David; Studer, Eveline; Steiner, Adrian.
In: Frontiers in Veterinary Science, Vol. 9, 1041215, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - “Non-healing” claw horn lesions in dairy cows
T2 - Clinical, histopathological and molecular biological characterization of four cases
AU - Alsaaod, Maher
AU - Weber, Jim
AU - Jensen, Tim
AU - Brandt, Sabine
AU - Gurtner, Corinne
AU - Devaux, David
AU - Studer, Eveline
AU - Steiner, Adrian
N1 - Publisher Copyright: Copyright © 2022 Alsaaod, Weber, Jensen, Brandt, Gurtner, Devaux, Studer and Steiner.
PY - 2022
Y1 - 2022
N2 - The increasing prevalence of bovine digital dermatitis (BDD) contributes to a higher occurrence of secondary infections of exposed corium with Treponema spp. in bovine claws. “Non-healing” claw horn lesions (NHL) clinically resemble BDD lesions. They are severe, cause chronic lameness, and may persist for several months. They poorly respond to standard treatments of BDD and represent a serious welfare issue. In this study, four cases of NHL were classified clinically either as BDD-associated axial horn fissures (BDD-HFA; n = 3) or BDD-associated sole ulcer (BDD-SU; n = 1). In all four cases, pronounced multifocal keratinolysis of the stratum corneum, ulceration, and severe chronic lymphoplasmacytic perivascular to interstitial dermatitis were observed. All lesional samples tested positive for Treponema spp., Fusobacterium (F.) necrophorum, and Porphyromonas (P.) levii by PCRs. BDD-HFA lesions contained Treponema pedis as revealed by genetic identities of 93, 99, and 100%. Treponemes in the BDD-SU lesion were 94% homologous to Treponema phylotype PT3. Fluorescent in situ hybridization (FISH) revealed extensive epidermal infiltration by treponemes that made up > 90% of the total bacterial population in all four lesions. FISH also tested positive for P. levii and negative for F. necrophorum in all four cases, whilst only one BDD-HFA contained Dichelobacter nodosus. Our data point to BDD-associated treponemes and P. levii constituting potential etiological agents in the development of “non-healing” claw horn lesions in cattle.
AB - The increasing prevalence of bovine digital dermatitis (BDD) contributes to a higher occurrence of secondary infections of exposed corium with Treponema spp. in bovine claws. “Non-healing” claw horn lesions (NHL) clinically resemble BDD lesions. They are severe, cause chronic lameness, and may persist for several months. They poorly respond to standard treatments of BDD and represent a serious welfare issue. In this study, four cases of NHL were classified clinically either as BDD-associated axial horn fissures (BDD-HFA; n = 3) or BDD-associated sole ulcer (BDD-SU; n = 1). In all four cases, pronounced multifocal keratinolysis of the stratum corneum, ulceration, and severe chronic lymphoplasmacytic perivascular to interstitial dermatitis were observed. All lesional samples tested positive for Treponema spp., Fusobacterium (F.) necrophorum, and Porphyromonas (P.) levii by PCRs. BDD-HFA lesions contained Treponema pedis as revealed by genetic identities of 93, 99, and 100%. Treponemes in the BDD-SU lesion were 94% homologous to Treponema phylotype PT3. Fluorescent in situ hybridization (FISH) revealed extensive epidermal infiltration by treponemes that made up > 90% of the total bacterial population in all four lesions. FISH also tested positive for P. levii and negative for F. necrophorum in all four cases, whilst only one BDD-HFA contained Dichelobacter nodosus. Our data point to BDD-associated treponemes and P. levii constituting potential etiological agents in the development of “non-healing” claw horn lesions in cattle.
KW - bovine digital dermatitis
KW - dairy cow
KW - fluorescent in situ hybridization
KW - PCR
KW - treponemes
U2 - 10.3389/fvets.2022.1041215
DO - 10.3389/fvets.2022.1041215
M3 - Journal article
C2 - 36337205
AN - SCOPUS:85141361451
VL - 9
JO - Frontiers in Veterinary Science
JF - Frontiers in Veterinary Science
SN - 2297-1769
M1 - 1041215
ER -
ID: 337600134