Role of ion channels in human induced pluripotent stem cells-derived cardiomyocytes
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Standard
Role of ion channels in human induced pluripotent stem cells-derived cardiomyocytes. / Treat, Jacqueline A.; Jankova, Michelle; Calloe, Kirstine; Cordeiro, Jonathan M.
Molecular Players in iPSC Technology. Vol. 12 Academic Press, 2022. p. 219-248.Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - CHAP
T1 - Role of ion channels in human induced pluripotent stem cells-derived cardiomyocytes
AU - Treat, Jacqueline A.
AU - Jankova, Michelle
AU - Calloe, Kirstine
AU - Cordeiro, Jonathan M.
N1 - Publisher Copyright: © 2022 Elsevier Inc. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Human induced pluripotent stem cells directed to the cardiac lineage (hiPSC-CMs) are used in many platforms such as generating models of human genetic diseases and cardiac safety pharmacology whereby compounds bound for regulatory submission are tested on hiPSC-CMs to determine the drug effect on ion channels and action potentials. The cardiac action potential (AP) is an important physiological parameter: (1) the AP initiates excitation in the heart, (2) it modulates the refractory period due to a long AP duration, and (3) associated with each AP is a contraction. Alterations in the expression or gating of ion channels will change the time- and/or voltage-dependent properties and can have marked effects on the AP waveform. The electrophysiological immaturity of hiPSC-CM suggests caution when translating the results to the adult phenotype. This chapter will highlight the electrophysiological similarities and differences of the hiPSC myocyte compared to adult myocytes. We will first contrast AP waveform in hiPSC-CMs and adult ventricle and explore the underlying ionic and molecular basis for these differences. Finally, we will explore strategies employed by various laboratories to potentially improve the maturity of hiPSC-CMs.
AB - Human induced pluripotent stem cells directed to the cardiac lineage (hiPSC-CMs) are used in many platforms such as generating models of human genetic diseases and cardiac safety pharmacology whereby compounds bound for regulatory submission are tested on hiPSC-CMs to determine the drug effect on ion channels and action potentials. The cardiac action potential (AP) is an important physiological parameter: (1) the AP initiates excitation in the heart, (2) it modulates the refractory period due to a long AP duration, and (3) associated with each AP is a contraction. Alterations in the expression or gating of ion channels will change the time- and/or voltage-dependent properties and can have marked effects on the AP waveform. The electrophysiological immaturity of hiPSC-CM suggests caution when translating the results to the adult phenotype. This chapter will highlight the electrophysiological similarities and differences of the hiPSC myocyte compared to adult myocytes. We will first contrast AP waveform in hiPSC-CMs and adult ventricle and explore the underlying ionic and molecular basis for these differences. Finally, we will explore strategies employed by various laboratories to potentially improve the maturity of hiPSC-CMs.
KW - Action potentials
KW - Calcium current
KW - Cardiomyocytes
KW - Depolarization
KW - Electrophysiology
KW - Ion channel currents
KW - Potassium current
KW - Repolarization
KW - Sodium current
KW - Stem cells
U2 - 10.1016/B978-0-323-90059-1.00001-4
DO - 10.1016/B978-0-323-90059-1.00001-4
M3 - Book chapter
AN - SCOPUS:85128091929
VL - 12
SP - 219
EP - 248
BT - Molecular Players in iPSC Technology
PB - Academic Press
ER -
ID: 304359365