TY - JOUR

T1 - Evaluation of an antibody detecting point of care test for diagnosis of Taenia solium cysticercosis in a Zambian rural community

T2 - A prospective diagnostic accuracy study

AU - Mubanga, Chishimba

AU - Van Damme, Inge

AU - Trevisan, Chiara

AU - Schmidt, Veronika

AU - Phiri, Isaac K

AU - Zulu, Gideon

AU - Noh, John

AU - Handali, Sukwan

AU - Mambo, Richard

AU - Chembensofu, Mwelwa

AU - Masuku, Maxwell

AU - Reynders, Dries

AU - Jansen, Famke

AU - Bottieau, Emmanuel

AU - Magnussen, Pascal

AU - Winkler, Andrea S

AU - Dorny, Pierre

AU - Mwape, Kabemba E

AU - Gabriël, Sarah

PY - 2021

Y1 - 2021

N2 - The lack of cheap, easy-to-use, rapid diagnostic tests has led to the development of several rapid diagnostic tests for cysticercosis. The new prototype two-strip, Taenia solium point of care test (TS POC) detects antibodies against taeniosis (TS POC T) and cysticercosis (TS POC CC). This study evaluated the diagnostic performance of the TS POC CC in the Sinda district in eastern Zambia. A sample of 1254 participants was recruited and tested with the TS POC. Out of the 1249 participants with a valid TS POC result, 177 (14%) tested positive while 1072 (86%) tested negative. All individuals with a positive TS POC and a subset of negative TS POC participants were selected for serum sampling, and were subjected to the recombinant glycoprotein T24H enzyme-linked immunoelectrotransfer blot (rT24H EITB) and the serum B60/158 (serum Ag) enzyme-linked immunosorbent assay (Ag ELISA). Performance characteristics were estimated using a Bayesian approach with probabilistic constraints. Based on 255 complete cases, the estimated sensitivity and specificity of the TS POC CC test were 35% (95% CI: 14-63%) and 87% (95% CI: 83-90%), respectively. The diagnostic performance needs to be improved, possibly by titrating antigen and other reagents' concentration in the strip to produce a performance similar to existing cysticercosis tests such as the rT24H EITB.

AB - The lack of cheap, easy-to-use, rapid diagnostic tests has led to the development of several rapid diagnostic tests for cysticercosis. The new prototype two-strip, Taenia solium point of care test (TS POC) detects antibodies against taeniosis (TS POC T) and cysticercosis (TS POC CC). This study evaluated the diagnostic performance of the TS POC CC in the Sinda district in eastern Zambia. A sample of 1254 participants was recruited and tested with the TS POC. Out of the 1249 participants with a valid TS POC result, 177 (14%) tested positive while 1072 (86%) tested negative. All individuals with a positive TS POC and a subset of negative TS POC participants were selected for serum sampling, and were subjected to the recombinant glycoprotein T24H enzyme-linked immunoelectrotransfer blot (rT24H EITB) and the serum B60/158 (serum Ag) enzyme-linked immunosorbent assay (Ag ELISA). Performance characteristics were estimated using a Bayesian approach with probabilistic constraints. Based on 255 complete cases, the estimated sensitivity and specificity of the TS POC CC test were 35% (95% CI: 14-63%) and 87% (95% CI: 83-90%), respectively. The diagnostic performance needs to be improved, possibly by titrating antigen and other reagents' concentration in the strip to produce a performance similar to existing cysticercosis tests such as the rT24H EITB.

U2 - 10.3390/diagnostics11112121

DO - 10.3390/diagnostics11112121

M3 - Journal article

C2 - 34829468

VL - 11

JO - Diagnostics

JF - Diagnostics

SN - 2075-4418

IS - 11

M1 - 2121

ER -