In vitro hatching of Trichuris suis eggs

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Standard

In vitro hatching of Trichuris suis eggs. / Vejzagic, Nermina; Thamsborg, Stig Milan; Kringel, Helene; Roepstorff, Allan; Bruun, Johan Musaeus; Kapel, Christian Moliin Outzen.

I: Parasitology Research, Bind 114, Nr. 7, 2015, s. 2705-2714.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Vejzagic, N, Thamsborg, SM, Kringel, H, Roepstorff, A, Bruun, JM & Kapel, CMO 2015, 'In vitro hatching of Trichuris suis eggs', Parasitology Research, bind 114, nr. 7, s. 2705-2714. https://doi.org/10.1007/s00436-015-4476-1

APA

Vejzagic, N., Thamsborg, S. M., Kringel, H., Roepstorff, A., Bruun, J. M., & Kapel, C. M. O. (2015). In vitro hatching of Trichuris suis eggs. Parasitology Research, 114(7), 2705-2714. https://doi.org/10.1007/s00436-015-4476-1

Vancouver

Vejzagic N, Thamsborg SM, Kringel H, Roepstorff A, Bruun JM, Kapel CMO. In vitro hatching of Trichuris suis eggs. Parasitology Research. 2015;114(7):2705-2714. https://doi.org/10.1007/s00436-015-4476-1

Author

Vejzagic, Nermina ; Thamsborg, Stig Milan ; Kringel, Helene ; Roepstorff, Allan ; Bruun, Johan Musaeus ; Kapel, Christian Moliin Outzen. / In vitro hatching of Trichuris suis eggs. I: Parasitology Research. 2015 ; Bind 114, Nr. 7. s. 2705-2714.

Bibtex

@article{549285657ee84373b40a894f30c6a935,
title = "In vitro hatching of Trichuris suis eggs",
abstract = "Eggs of the pig whipworm, Trichuris suis ova (TSO), are currently tested in human clinical trials for their potential immunomodulatory capacity. The biological potency of TSO (egg viability and infectivity) is traditionally assessed in G{\"o}ttingen minipigs as the establishment of intestinal larvae after inoculation with a known number of eggs. To minimize testing in animal models, development of an in vitro egg hatching assay is proposed as a reliable, cost-effective, and a faster alternative to test the egg viability. The present study aimed to investigate the influence of different chemical, physical, and biological factors on egg hatching. Thus, in a series of experiments and in different combinations, the eggs were stimulated with glass beads, artificial gastric juice, bile salt and trypsin solution, fermentation gut medium, or stimulated with mucosal scrapings from the ileum and the large intestine of the infected and uninfected G{\"o}ttingen minipig. Mechanical stimulation with glass beads presented a simple and reproducible method for egg hatching. However, incubation of eggs with mucosal scrapings from the ileum, caecum, and colon for 24 h at 38 °C significantly increased hatching.",
author = "Nermina Vejzagic and Thamsborg, {Stig Milan} and Helene Kringel and Allan Roepstorff and Bruun, {Johan Musaeus} and Kapel, {Christian Moliin Outzen}",
year = "2015",
doi = "10.1007/s00436-015-4476-1",
language = "English",
volume = "114",
pages = "2705--2714",
journal = "Parasitology Research",
issn = "0932-0113",
publisher = "Springer",
number = "7",

}

RIS

TY - JOUR

T1 - In vitro hatching of Trichuris suis eggs

AU - Vejzagic, Nermina

AU - Thamsborg, Stig Milan

AU - Kringel, Helene

AU - Roepstorff, Allan

AU - Bruun, Johan Musaeus

AU - Kapel, Christian Moliin Outzen

PY - 2015

Y1 - 2015

N2 - Eggs of the pig whipworm, Trichuris suis ova (TSO), are currently tested in human clinical trials for their potential immunomodulatory capacity. The biological potency of TSO (egg viability and infectivity) is traditionally assessed in Göttingen minipigs as the establishment of intestinal larvae after inoculation with a known number of eggs. To minimize testing in animal models, development of an in vitro egg hatching assay is proposed as a reliable, cost-effective, and a faster alternative to test the egg viability. The present study aimed to investigate the influence of different chemical, physical, and biological factors on egg hatching. Thus, in a series of experiments and in different combinations, the eggs were stimulated with glass beads, artificial gastric juice, bile salt and trypsin solution, fermentation gut medium, or stimulated with mucosal scrapings from the ileum and the large intestine of the infected and uninfected Göttingen minipig. Mechanical stimulation with glass beads presented a simple and reproducible method for egg hatching. However, incubation of eggs with mucosal scrapings from the ileum, caecum, and colon for 24 h at 38 °C significantly increased hatching.

AB - Eggs of the pig whipworm, Trichuris suis ova (TSO), are currently tested in human clinical trials for their potential immunomodulatory capacity. The biological potency of TSO (egg viability and infectivity) is traditionally assessed in Göttingen minipigs as the establishment of intestinal larvae after inoculation with a known number of eggs. To minimize testing in animal models, development of an in vitro egg hatching assay is proposed as a reliable, cost-effective, and a faster alternative to test the egg viability. The present study aimed to investigate the influence of different chemical, physical, and biological factors on egg hatching. Thus, in a series of experiments and in different combinations, the eggs were stimulated with glass beads, artificial gastric juice, bile salt and trypsin solution, fermentation gut medium, or stimulated with mucosal scrapings from the ileum and the large intestine of the infected and uninfected Göttingen minipig. Mechanical stimulation with glass beads presented a simple and reproducible method for egg hatching. However, incubation of eggs with mucosal scrapings from the ileum, caecum, and colon for 24 h at 38 °C significantly increased hatching.

U2 - 10.1007/s00436-015-4476-1

DO - 10.1007/s00436-015-4476-1

M3 - Journal article

C2 - 26008635

VL - 114

SP - 2705

EP - 2714

JO - Parasitology Research

JF - Parasitology Research

SN - 0932-0113

IS - 7

ER -

ID: 144794796