Molecular epidemiology of Cryptosporidium spp. in calves in Estonia: High prevalence of Cryptosporidium parvum shedding and 10 subtypes identified
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Molecular epidemiology of Cryptosporidium spp. in calves in Estonia : High prevalence of Cryptosporidium parvum shedding and 10 subtypes identified. / Santoro, Azzurra; Dorbek-Kolin, Elisabeth; Jeremejeva, Julia; Tummeleht, Lea; Orro, Toomas; Jokelainen, Pikka; Lassen, Brian.
I: Parasitology, Bind 146, Nr. 2, 2019, s. 261-267.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Molecular epidemiology of Cryptosporidium spp. in calves in Estonia
T2 - High prevalence of Cryptosporidium parvum shedding and 10 subtypes identified
AU - Santoro, Azzurra
AU - Dorbek-Kolin, Elisabeth
AU - Jeremejeva, Julia
AU - Tummeleht, Lea
AU - Orro, Toomas
AU - Jokelainen, Pikka
AU - Lassen, Brian
PY - 2019
Y1 - 2019
N2 - We investigated the molecular epidemiology of Cryptosporidium spp. in Estonia by testing fecal samples from 486 calves aged <2 months, raised on 53 cattle farms, for the presence of Cryptosporidium DNA. The parasites were identified and characterized by sequencing of the 18S rRNA gene and of the 60 kDa glycoprotein (gp60) gene. Moreover, using a questionnaire, we surveyed factors that could be relevant for animal-to-human and human-to-animal transmission of Cryptosporidium spp. on the farms. Cryptosporidium spp. were shed by 23% of the investigated calves and at least one shedding calf was found on 66% of the farms. Cryptosporidium parvum was the most common species shed, while C. bovis and C. ryanae were also detected. More than half of the calves aged 8-14 days shed C. parvum. Nine previously described C. parvum subtypes (IIaA14G1R1, IIaA16G1R1, IIaA17G1R1, IIaA18G1R1, IIaA19G1R1, IIaA20G1R1, IIaA21G1R1, IIaA22G1R1 and IIaA16G2R1) and an apparently novel subtype IIlA21R2 were found. Calves from farms that reported spreading manure on fields during spring had 10 times higher odds to shed Cryptosporidium spp. in their feces than calves from farms that did not. Calves aged 8-14 days had higher odds to shed IIa18G1R1 as well as IIaA16G1R1 than younger calves.
AB - We investigated the molecular epidemiology of Cryptosporidium spp. in Estonia by testing fecal samples from 486 calves aged <2 months, raised on 53 cattle farms, for the presence of Cryptosporidium DNA. The parasites were identified and characterized by sequencing of the 18S rRNA gene and of the 60 kDa glycoprotein (gp60) gene. Moreover, using a questionnaire, we surveyed factors that could be relevant for animal-to-human and human-to-animal transmission of Cryptosporidium spp. on the farms. Cryptosporidium spp. were shed by 23% of the investigated calves and at least one shedding calf was found on 66% of the farms. Cryptosporidium parvum was the most common species shed, while C. bovis and C. ryanae were also detected. More than half of the calves aged 8-14 days shed C. parvum. Nine previously described C. parvum subtypes (IIaA14G1R1, IIaA16G1R1, IIaA17G1R1, IIaA18G1R1, IIaA19G1R1, IIaA20G1R1, IIaA21G1R1, IIaA22G1R1 and IIaA16G2R1) and an apparently novel subtype IIlA21R2 were found. Calves from farms that reported spreading manure on fields during spring had 10 times higher odds to shed Cryptosporidium spp. in their feces than calves from farms that did not. Calves aged 8-14 days had higher odds to shed IIa18G1R1 as well as IIaA16G1R1 than younger calves.
KW - Baltic country
KW - bovine
KW - cattle
KW - cryptosporidiosis
KW - Cryptosporidium parvum
KW - environment
KW - molecular epidemiology
KW - One Health
U2 - 10.1017/S0031182018001348
DO - 10.1017/S0031182018001348
M3 - Journal article
C2 - 30086806
AN - SCOPUS:85052711986
VL - 146
SP - 261
EP - 267
JO - Parasitology
JF - Parasitology
SN - 0031-1820
IS - 2
ER -
ID: 202285539