TY - JOUR

T1 - Demonstration of airborne transmission of Actinobacillus pleuropneumoniae serotype 2 between simulated pig units located at close range

AU - Kristensen, C. S.

AU - Angen, null

AU - Andreasen, M.

AU - Takai, H.

AU - Nielsen, J. P.

AU - Jorsal, S. E.

N1 - Funding Information: The present study was financed by Research Centre for the Management of Animal Production and Health (CEPROS), Foulum, DK-8830 Tjele, CEP97-15. Thanks to Roald Koudahl, Ole Hjuler Sørense, Peter Ravn, Tamara Plambeck, Jannie Jensen, Peter Juul Kristensen, Gitte Drejer and Hasse Poulsen for technical assistance.

PY - 2004/3/5

Y1 - 2004/3/5

N2 - Airborne transmission of Actinobacillus pleuropneumoniae was studied as the percentage of air needed to establish airborne transmission from an infected pig unit into a neighbouring non-infected pig unit. The experiment was carried out in two containers constructed as pig units, placed 1m apart and connected by pipes. By manipulating the air pressure in the two units, the amount of ventilation air transferred from the infected pigs (unit A) to the non-infected pigs (unit B) was controlled and measured. In three experiments, between 48 and 50 specific pathogen free-pigs were randomly assigned to each of the two units. In unit A, five pigs (experiment 1) or eight pigs (experiments 2 and 3) were inoculated with A. pleuropneumoniae serotype 2. In experiments 1 and 3, 10% of the air was transferred from unit A to B; in experiment 2, 70% of the air was transferred. In the non-infected unit (B), 36% of the pigs seroconverted during experiment 2 (70% air transfer), whereas none of the pigs seroconverted in experiments 1 and 3 (10% air transfer). As air transmission between closely located pig units has been estimated to be less than 2% under field conditions, these results indicate that airborne transmission of A. pleuropneumoniae serotype 2 between closely located pig units is rare.

AB - Airborne transmission of Actinobacillus pleuropneumoniae was studied as the percentage of air needed to establish airborne transmission from an infected pig unit into a neighbouring non-infected pig unit. The experiment was carried out in two containers constructed as pig units, placed 1m apart and connected by pipes. By manipulating the air pressure in the two units, the amount of ventilation air transferred from the infected pigs (unit A) to the non-infected pigs (unit B) was controlled and measured. In three experiments, between 48 and 50 specific pathogen free-pigs were randomly assigned to each of the two units. In unit A, five pigs (experiment 1) or eight pigs (experiments 2 and 3) were inoculated with A. pleuropneumoniae serotype 2. In experiments 1 and 3, 10% of the air was transferred from unit A to B; in experiment 2, 70% of the air was transferred. In the non-infected unit (B), 36% of the pigs seroconverted during experiment 2 (70% air transfer), whereas none of the pigs seroconverted in experiments 1 and 3 (10% air transfer). As air transmission between closely located pig units has been estimated to be less than 2% under field conditions, these results indicate that airborne transmission of A. pleuropneumoniae serotype 2 between closely located pig units is rare.

KW - Actinobacillus pleuropneumoniae

KW - Airborne transmission

KW - Clinical observation

KW - Pig-bacteria

KW - Serology

UR - http://www.scopus.com/inward/record.url?scp=1242283859&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2003.10.026

DO - 10.1016/j.vetmic.2003.10.026

M3 - Journal article

C2 - 15036533

AN - SCOPUS:1242283859

VL - 98

SP - 243

EP - 249

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -