Antimicrobial stewardship initiatives are widely regarded as a cornerstone for ameliorating the global healthimpact of antimicrobial resistance. Within companion animal health, such efforts have largely focused ondevelopment and dissemination of antimicrobial stewardship guidelines (ASGs). However, there have been fewattempts to understand veterinarian attitudes towards and knowledge of ASGs or to determine how awarenessregarding ASGs might best be increased. An online survey regarding ASGs was formulated for veterinarians whotreat companion animals. The survey was distributed across 46 European and associated countries between 12January and 30 June, 2022. In total, 2271 surveys were completed, with 64.9% of respondents (n = 1474)reporting awareness and usage of at least one ASG. Respondents from countries with greater awareness of ASGstended to report more appropriate use of antimicrobials (Spearman’s rank coefficient = 0.6084, P ≤ 0.001), withrespondents from countries with country-specific ASGs tending to score highest across both awareness andappropriate use domains. Respondents prioritised guidance around antimicrobial choice (82.0%, n = 1863),duration of treatment (66.0%, n = 1499), and dosage (51.9%, n = 1179) for inclusion in future ASGs, with 78.0%(n = 1776) of respondents preferring ASGs to be integrated into their patient management system. Awareness ofASGs and their use in companion animal veterinary practice appears to be greater than previously reported, withrespondents tending to report antimicrobial prescription decision making broadly in line with current clinicalrecommendations. However, further initiatives aimed at maximising accessibility to ASGs both within countriesand individual veterinary practices are recommended.