Bacteriostatic and Haemolytic Activities of Extracts and Compounds of Commiphora swynnertonii
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Bacteriostatic and Haemolytic Activities of Extracts and Compounds of Commiphora swynnertonii. / Msengwa, Zaituni; Credo, David; Mafuru, Magesa; Mwesongo, James; Mabiki, Faith Philemon; Mwang’onde, Beda John; Mtambo, Madundo Mkumbukwa; Kusiluka, Lughano Jeremy; Mdegela, Robinson Hammerthon; Olsen, John Elmerdahl.
I: Research Journal of Pharmacognosy, Bind 10, Nr. 2, 2023, s. 47-56.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Bacteriostatic and Haemolytic Activities of Extracts and Compounds of Commiphora swynnertonii
AU - Msengwa, Zaituni
AU - Credo, David
AU - Mafuru, Magesa
AU - Mwesongo, James
AU - Mabiki, Faith Philemon
AU - Mwang’onde, Beda John
AU - Mtambo, Madundo Mkumbukwa
AU - Kusiluka, Lughano Jeremy
AU - Mdegela, Robinson Hammerthon
AU - Olsen, John Elmerdahl
N1 - Publisher Copyright: © 2023. Open access.
PY - 2023
Y1 - 2023
N2 - Background and objective: Commiphora swynnertonii (Pax) is used in traditional medicine to treat infectious diseases. Previous studies have reported antimicrobial activity of this plant; however, the activity of compounds that are present in extracts of this plant has not been thoroughly documented. Likewise, the primary mode of action (bactericidal or bacteriostatic) and the possible toxicity on red blood cells have not been reported. Methods: Extracts of leaves, whole root, root bark, root wood, whole stem, stem bark and stem wood, were produced using hexane, dichloromethane, methanol and water. Cold and hot extraction methods were employed. Antibacterial activity of extracts was tested against selected medically important Gram-positive and Gram-negative bacteria by growth inhibition, minimum inhibitory concentrations and time kill assays. Moreover, haemolytic activity against sheep red blood cells was determined in vitro. Results: The hexane extracts of whole root and root bark, methanol extracts of root wood, and dichloromethane extracts from the leaves of C. swynnertonii inhibited the growth of S. aureus. MIC values for the extracts and compounds, indicated moderate activity against Gram-positive bacteria (Staphylococcus aureus, Streptococci species and Enterococci species) while the activity against Gram-negative bacteria (Escherichia coli, Klebsiella species, Pseudomonas aeruginosa, Salmonella species, Shigella sonnei and Yersinia enterocolitica) was weak. Time kill profiles showed the extracts have bacteriostatic activity against S. aureus, and low haemolytic effect, except for extracts of whole root and leaves at the concentration of 1000 µg/mL. Conclusion: Extracts of C. swynnertonii showed bacteriostatic activity against Gram-positive bacteria with low toxicity on red blood cells.
AB - Background and objective: Commiphora swynnertonii (Pax) is used in traditional medicine to treat infectious diseases. Previous studies have reported antimicrobial activity of this plant; however, the activity of compounds that are present in extracts of this plant has not been thoroughly documented. Likewise, the primary mode of action (bactericidal or bacteriostatic) and the possible toxicity on red blood cells have not been reported. Methods: Extracts of leaves, whole root, root bark, root wood, whole stem, stem bark and stem wood, were produced using hexane, dichloromethane, methanol and water. Cold and hot extraction methods were employed. Antibacterial activity of extracts was tested against selected medically important Gram-positive and Gram-negative bacteria by growth inhibition, minimum inhibitory concentrations and time kill assays. Moreover, haemolytic activity against sheep red blood cells was determined in vitro. Results: The hexane extracts of whole root and root bark, methanol extracts of root wood, and dichloromethane extracts from the leaves of C. swynnertonii inhibited the growth of S. aureus. MIC values for the extracts and compounds, indicated moderate activity against Gram-positive bacteria (Staphylococcus aureus, Streptococci species and Enterococci species) while the activity against Gram-negative bacteria (Escherichia coli, Klebsiella species, Pseudomonas aeruginosa, Salmonella species, Shigella sonnei and Yersinia enterocolitica) was weak. Time kill profiles showed the extracts have bacteriostatic activity against S. aureus, and low haemolytic effect, except for extracts of whole root and leaves at the concentration of 1000 µg/mL. Conclusion: Extracts of C. swynnertonii showed bacteriostatic activity against Gram-positive bacteria with low toxicity on red blood cells.
KW - antibacterial
KW - Commiphora swynnertonii
KW - medicinal plant
KW - red blood cells
U2 - 10.22127/RJP.2023.370231.2009
DO - 10.22127/RJP.2023.370231.2009
M3 - Journal article
AN - SCOPUS:85151987327
VL - 10
SP - 47
EP - 56
JO - Research Journal of Pharmacognosy
JF - Research Journal of Pharmacognosy
SN - 2345-4458
IS - 2
ER -
ID: 343338296