Identification and characterization of a spreadable IncI1 plasmid harbouring a blaCTX-M-15 gene in an Italian human isolate of Salmonella serovar Napoli

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Identification and characterization of a spreadable IncI1 plasmid harbouring a blaCTX-M-15 gene in an Italian human isolate of Salmonella serovar Napoli. / Petrin, Sara; Orsini, Massimiliano; Mastrorilli, Eleonora; Longo, Alessandra; Cozza, Debora; Olsen, John E.; Ricci, Antonia; Losasso, Carmen; Barco, Lisa.

I: Plasmid, Bind 114, 102566, 2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Petrin, S, Orsini, M, Mastrorilli, E, Longo, A, Cozza, D, Olsen, JE, Ricci, A, Losasso, C & Barco, L 2021, 'Identification and characterization of a spreadable IncI1 plasmid harbouring a blaCTX-M-15 gene in an Italian human isolate of Salmonella serovar Napoli', Plasmid, bind 114, 102566. https://doi.org/10.1016/j.plasmid.2021.102566

APA

Petrin, S., Orsini, M., Mastrorilli, E., Longo, A., Cozza, D., Olsen, J. E., Ricci, A., Losasso, C., & Barco, L. (2021). Identification and characterization of a spreadable IncI1 plasmid harbouring a blaCTX-M-15 gene in an Italian human isolate of Salmonella serovar Napoli. Plasmid, 114, [102566]. https://doi.org/10.1016/j.plasmid.2021.102566

Vancouver

Petrin S, Orsini M, Mastrorilli E, Longo A, Cozza D, Olsen JE o.a. Identification and characterization of a spreadable IncI1 plasmid harbouring a blaCTX-M-15 gene in an Italian human isolate of Salmonella serovar Napoli. Plasmid. 2021;114. 102566. https://doi.org/10.1016/j.plasmid.2021.102566

Author

Petrin, Sara ; Orsini, Massimiliano ; Mastrorilli, Eleonora ; Longo, Alessandra ; Cozza, Debora ; Olsen, John E. ; Ricci, Antonia ; Losasso, Carmen ; Barco, Lisa. / Identification and characterization of a spreadable IncI1 plasmid harbouring a blaCTX-M-15 gene in an Italian human isolate of Salmonella serovar Napoli. I: Plasmid. 2021 ; Bind 114.

Bibtex

@article{911ff14c06844f058a201b0031e57435,
title = "Identification and characterization of a spreadable IncI1 plasmid harbouring a blaCTX-M-15 gene in an Italian human isolate of Salmonella serovar Napoli",
abstract = "Salmonella enterica subsp. enterica serovar Napoli (S. Napoli) ranks among the top serovars causing human infections in Italy, although not common in other European countries. Isolates are generally pan-susceptible or resistant to aminoglycosides only, however data on antimicrobial resistance genes in strains of S. Napoli are limited. Recently an isolate encoding resistance to third generation cephalosporins was reported. This study aimed to characterize plasmid-encoded cephalosporin resistance due to the blaCTX-M-15 gene in a human S. Napoli isolate in Italy, and to investigate plasmid stability over time. S. Napoli 16/174478 was confirmed to be ESBL-producing. The blaCTX-M-15 gene was shown to be located on an IncI1α plasmid of 90,272 bp (50.03 GC%) encoding for 107 coding sequences (CDS). The plasmid was successfully transferred by conjugation to an E. coli 1816 recipient strain (conjugation frequency 3.9 × 10−2 transconjugants per donor). Transconjugants were confirmed to carry the IncI1α plasmid, and to be ESBL-producing strains as well. Moreover, transconjugant colonies maintained the plasmid for up to 10 passages. The identification of S. Napoli isolates able to produce ESBLs is of great concern, as this pathogen is frequently associated with invasive infections and a higher risk of bacteraemia, and its reservoir has not yet been clearly identified.",
keywords = "Antimicrobial resistance genes, ESBLs, Persistence, Plasmid, Salmonella Napoli, Third-generation cephalosporins",
author = "Sara Petrin and Massimiliano Orsini and Eleonora Mastrorilli and Alessandra Longo and Debora Cozza and Olsen, {John E.} and Antonia Ricci and Carmen Losasso and Lisa Barco",
year = "2021",
doi = "10.1016/j.plasmid.2021.102566",
language = "English",
volume = "114",
journal = "Plasmid",
issn = "0147-619X",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Identification and characterization of a spreadable IncI1 plasmid harbouring a blaCTX-M-15 gene in an Italian human isolate of Salmonella serovar Napoli

AU - Petrin, Sara

AU - Orsini, Massimiliano

AU - Mastrorilli, Eleonora

AU - Longo, Alessandra

AU - Cozza, Debora

AU - Olsen, John E.

AU - Ricci, Antonia

AU - Losasso, Carmen

AU - Barco, Lisa

PY - 2021

Y1 - 2021

N2 - Salmonella enterica subsp. enterica serovar Napoli (S. Napoli) ranks among the top serovars causing human infections in Italy, although not common in other European countries. Isolates are generally pan-susceptible or resistant to aminoglycosides only, however data on antimicrobial resistance genes in strains of S. Napoli are limited. Recently an isolate encoding resistance to third generation cephalosporins was reported. This study aimed to characterize plasmid-encoded cephalosporin resistance due to the blaCTX-M-15 gene in a human S. Napoli isolate in Italy, and to investigate plasmid stability over time. S. Napoli 16/174478 was confirmed to be ESBL-producing. The blaCTX-M-15 gene was shown to be located on an IncI1α plasmid of 90,272 bp (50.03 GC%) encoding for 107 coding sequences (CDS). The plasmid was successfully transferred by conjugation to an E. coli 1816 recipient strain (conjugation frequency 3.9 × 10−2 transconjugants per donor). Transconjugants were confirmed to carry the IncI1α plasmid, and to be ESBL-producing strains as well. Moreover, transconjugant colonies maintained the plasmid for up to 10 passages. The identification of S. Napoli isolates able to produce ESBLs is of great concern, as this pathogen is frequently associated with invasive infections and a higher risk of bacteraemia, and its reservoir has not yet been clearly identified.

AB - Salmonella enterica subsp. enterica serovar Napoli (S. Napoli) ranks among the top serovars causing human infections in Italy, although not common in other European countries. Isolates are generally pan-susceptible or resistant to aminoglycosides only, however data on antimicrobial resistance genes in strains of S. Napoli are limited. Recently an isolate encoding resistance to third generation cephalosporins was reported. This study aimed to characterize plasmid-encoded cephalosporin resistance due to the blaCTX-M-15 gene in a human S. Napoli isolate in Italy, and to investigate plasmid stability over time. S. Napoli 16/174478 was confirmed to be ESBL-producing. The blaCTX-M-15 gene was shown to be located on an IncI1α plasmid of 90,272 bp (50.03 GC%) encoding for 107 coding sequences (CDS). The plasmid was successfully transferred by conjugation to an E. coli 1816 recipient strain (conjugation frequency 3.9 × 10−2 transconjugants per donor). Transconjugants were confirmed to carry the IncI1α plasmid, and to be ESBL-producing strains as well. Moreover, transconjugant colonies maintained the plasmid for up to 10 passages. The identification of S. Napoli isolates able to produce ESBLs is of great concern, as this pathogen is frequently associated with invasive infections and a higher risk of bacteraemia, and its reservoir has not yet been clearly identified.

KW - Antimicrobial resistance genes

KW - ESBLs

KW - Persistence

KW - Plasmid

KW - Salmonella Napoli

KW - Third-generation cephalosporins

U2 - 10.1016/j.plasmid.2021.102566

DO - 10.1016/j.plasmid.2021.102566

M3 - Journal article

C2 - 33582117

AN - SCOPUS:85101394758

VL - 114

JO - Plasmid

JF - Plasmid

SN - 0147-619X

M1 - 102566

ER -

ID: 257874918