Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy
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Staphylococcus aureus is the most common pathogen causing osteomyelitis (OM). The aim of this study was to explore the clonal complex (CC) distribution and the pattern of virulence determinants of S. aureus isolates from OM in Italy. Whole-genome sequencing was performed on 83 S. aureus isolates from OM cases in six hospitals. Antibiotic susceptibility tests showed that 30.1% of the isolates were methicillin-resistant S. aureus (MRSA). The most frequent CCs detected were CC22, CC5, CC8, CC30, and CC15, which represent the most common lineages circulating in Italian hospitals. MRSA were limited in the number of lineages (CC22, CC5, CC8, and CC1). Phylogenetic analysis followed the sequence type-CC groupings and revealed a non-uniform distribution of the isolates from the different hospitals. No significant difference in the mean number of virulence genes carried by MRSA or MSSA isolates was observed. Some virulence genes, namely cna, fib, fnbA, coa, lukD, lukE, sak, and tst, were correlated with the CC. However, different categories of virulence factors, such as adhesins, exoenzymes, and toxins, were frequently detected and unevenly distributed among all lineages. Indeed, each lineage carried a variable combination of virulence genes, likely reflecting functional redundancy, and arguing for the importance of those traits for the pathogenicity in OM. In conclusion, no specific genetic trait in the most frequent lineages could explain their high prevalence among OM isolates. Our findings highlight that CCs detected in OM isolates follow the epidemiology of S. aureus infections in the country. It is conceivable that any of the most common S. aureus CC can cause a variety of infections, including OM.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | 846167 |
| Tidsskrift | Frontiers in Microbiology |
| Vol/bind | 13 |
| ISSN | 1664-302X |
| DOI | |
| Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
The authors wish to thank Pierluigi Viale (Infectious Diseases Unit, Department for Integrated Infectious Risk Management, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Italy), Nicol? Rossi (Infectious Diseases Unit, Department for Integrated Infectious Risk Management, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Italy), Domenico Tigani (Department of Orthopaedic Surgery, Ospedale Maggiore, Bologna, Italy), Calogero Alfonso (Orthopedics Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Italy), Mauro Girolami (Bentivoglio Orthopaedic Ward, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy), Carlo Impallomeni (Orthopedics Unit, Imola Hospital, Bologna, Italy), Gian Maria Rossolini (Department of Experimental and Clinical Medicine, University of Florence, Italy), Domenico Andrea Campanacci (Department of Orthopaedic Oncology and Reconstructive Surgery, Careggi University Hospital, Firenze, Italy), Alberto Farese (Infectious and Tropical Diseases Unit, Careggi University Hospital, Florence, Italy) for their contribution in data collection. The valuable support of Fabio D?Ambrosio (Department of Infectious Diseases, Istituto Superiore di Sanit?, Rome, Italy) and the technical staff of the Unit of Clinical Microbiology, S. Agostino-Estense, Hospital Baggiovara, Modena, Italy is also acknowledged.
Funding Information:
This work was supported in part by the Excellence Departments grant (art. 1, commi 314–337 Legge 232/2016) to the Department of Science, Roma Tre University, and grant PRIN 2017 (Prot. 20177J5Y3P) to PV, both from the Italian Ministry of Education, University and Research (MIUR), and in part by the Italian Ministry of Health, Centro Controllo Malattie (CCM), 2019 project “Sostegno alla Sorveglianza delle Infezioni correlate all’assistenza anche a supporto del PNCAR.”
Publisher Copyright:
Copyright © 2022 Pimentel de Araujo, Pirolo, Monaco, Del Grosso, Ambretti, Lombardo, Cassetti, Gargiulo, Riccobono, Visca and Pantosti.
ID: 308152648