A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples

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Standard

A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples. / Rossen, L.; Holmstrøm, Kim; Olsen, J. E.

I: International Journal of Food Microbiology, Bind 14, Nr. 2, 11.1991, s. 145-151.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Rossen, L, Holmstrøm, K & Olsen, JE 1991, 'A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples', International Journal of Food Microbiology, bind 14, nr. 2, s. 145-151. https://doi.org/10.1016/0168-1605(91)90101-T

APA

Rossen, L., Holmstrøm, K., & Olsen, J. E. (1991). A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples. International Journal of Food Microbiology, 14(2), 145-151. https://doi.org/10.1016/0168-1605(91)90101-T

Vancouver

Rossen L, Holmstrøm K, Olsen JE. A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples. International Journal of Food Microbiology. 1991 nov.;14(2):145-151. https://doi.org/10.1016/0168-1605(91)90101-T

Author

Rossen, L. ; Holmstrøm, Kim ; Olsen, J. E. / A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples. I: International Journal of Food Microbiology. 1991 ; Bind 14, Nr. 2. s. 145-151.

Bibtex

@article{82dc93a403834502b37a6357873698a5,
title = "A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples",
abstract = "A rapid and simple assay has been developed which allows specific detection of Listeria monocytogenes within 3.5 h in cultures prepared from suspect food samples and propagated 48 h in selective medium. The assay is based on PCR technology, and uses a specific primer set derived from sequences located down-stream of the hlyA gene. The specificity of the primer set was confirmed by testing 115 L. monocytogenes, 14 L. innocua, 5 L. seeligeri and 4 L. ivanovii isolates. The assay was compared to standard microbiological tests and gave identical results for 83 food samples, including 32 positives. These field trials indicate that the assay developed provides an alternative detection system for L. monocytogenes in foods, which can be used by the food industry.",
keywords = "Detection, DNA-probe, Foods, Listeria monocytogenes",
author = "L. Rossen and Kim Holmstr{\o}m and Olsen, {J. E.}",
year = "1991",
month = nov,
doi = "10.1016/0168-1605(91)90101-T",
language = "English",
volume = "14",
pages = "145--151",
journal = "International Journal of Food Microbiology",
issn = "0168-1605",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples

AU - Rossen, L.

AU - Holmstrøm, Kim

AU - Olsen, J. E.

PY - 1991/11

Y1 - 1991/11

N2 - A rapid and simple assay has been developed which allows specific detection of Listeria monocytogenes within 3.5 h in cultures prepared from suspect food samples and propagated 48 h in selective medium. The assay is based on PCR technology, and uses a specific primer set derived from sequences located down-stream of the hlyA gene. The specificity of the primer set was confirmed by testing 115 L. monocytogenes, 14 L. innocua, 5 L. seeligeri and 4 L. ivanovii isolates. The assay was compared to standard microbiological tests and gave identical results for 83 food samples, including 32 positives. These field trials indicate that the assay developed provides an alternative detection system for L. monocytogenes in foods, which can be used by the food industry.

AB - A rapid and simple assay has been developed which allows specific detection of Listeria monocytogenes within 3.5 h in cultures prepared from suspect food samples and propagated 48 h in selective medium. The assay is based on PCR technology, and uses a specific primer set derived from sequences located down-stream of the hlyA gene. The specificity of the primer set was confirmed by testing 115 L. monocytogenes, 14 L. innocua, 5 L. seeligeri and 4 L. ivanovii isolates. The assay was compared to standard microbiological tests and gave identical results for 83 food samples, including 32 positives. These field trials indicate that the assay developed provides an alternative detection system for L. monocytogenes in foods, which can be used by the food industry.

KW - Detection

KW - DNA-probe

KW - Foods

KW - Listeria monocytogenes

UR - http://www.scopus.com/inward/record.url?scp=0025937803&partnerID=8YFLogxK

U2 - 10.1016/0168-1605(91)90101-T

DO - 10.1016/0168-1605(91)90101-T

M3 - Journal article

C2 - 1777383

AN - SCOPUS:0025937803

VL - 14

SP - 145

EP - 151

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

IS - 2

ER -

ID: 257697963