Assessment of implantable infusion pumps for continuous infusion of human insulin in rats: potential for group housing
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Assessment of implantable infusion pumps for continuous infusion of human insulin in rats: potential for group housing. / Jensen, Vivi Flou Hjorth; Molck, Anne-Marie; Martensson, Martin; Strid, Mette Aagaard; Chapman, Melissa; Lykkesfeldt, Jens; Bogh, Ingrid Bruck.
I: Laboratory Animals, Bind 51, Nr. 3, 2017, s. 273-283.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Assessment of implantable infusion pumps for continuous infusion of human insulin in rats: potential for group housing
AU - Jensen, Vivi Flou Hjorth
AU - Molck, Anne-Marie
AU - Martensson, Martin
AU - Strid, Mette Aagaard
AU - Chapman, Melissa
AU - Lykkesfeldt, Jens
AU - Bogh, Ingrid Bruck
PY - 2017
Y1 - 2017
N2 - Group housing is considered to be important for rats, which are highly sociable animals. Single housing may impact behaviour and levels of circulating stress hormones. Rats are typically used in the toxicological evaluation of insulin analogues. Human insulin (HI) is frequently used as a reference compound in these studies, and a comparator model of persistent exposure by HI infusion from external pumps has recently been developed to support toxicological evaluation of long-acting insulin analogues. However, this model requires single housing of the animals. Developing an insulin-infusion model which allows group housing would therefore greatly improve animal welfare. The aim of the present study was to investigate the suitability of implantable infusion pumps for HI infusion in group-housed rats. Group housing of rats implanted with a battery-driven pump proved to be possible. Intravenous infusion of HI lowered blood glucose levels persistently for two weeks, providing a comparator model for use in two-week repeated-dose toxicity studies with new long-acting insulin analogues, which allows group housing, and thereby increasing animal welfare compared with an external infusion model.
AB - Group housing is considered to be important for rats, which are highly sociable animals. Single housing may impact behaviour and levels of circulating stress hormones. Rats are typically used in the toxicological evaluation of insulin analogues. Human insulin (HI) is frequently used as a reference compound in these studies, and a comparator model of persistent exposure by HI infusion from external pumps has recently been developed to support toxicological evaluation of long-acting insulin analogues. However, this model requires single housing of the animals. Developing an insulin-infusion model which allows group housing would therefore greatly improve animal welfare. The aim of the present study was to investigate the suitability of implantable infusion pumps for HI infusion in group-housed rats. Group housing of rats implanted with a battery-driven pump proved to be possible. Intravenous infusion of HI lowered blood glucose levels persistently for two weeks, providing a comparator model for use in two-week repeated-dose toxicity studies with new long-acting insulin analogues, which allows group housing, and thereby increasing animal welfare compared with an external infusion model.
KW - reduction
KW - refinement
KW - infusion
KW - rats
KW - human insulin
U2 - 10.1177/0023677216660740
DO - 10.1177/0023677216660740
M3 - Journal article
C2 - 27465034
VL - 51
SP - 273
EP - 283
JO - Laboratory Animals
JF - Laboratory Animals
SN - 0023-6772
IS - 3
ER -
ID: 179526539