Effect of soybean oil and fish oil on lipid-related transcripts in subcutaneous adipose tissue of dairy cows
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Effect of soybean oil and fish oil on lipid-related transcripts in subcutaneous adipose tissue of dairy cows. / Vargas-Bello-Pérez, Einar; Bionaz, Massimo; Garrido-Sartore, Macarena; Cancino-Padilla, Nathaly; Morales, María Sol; Romero, Jaime; Leskinen, Heidi; Garnsworthy, Philip C.; Loor, Juan J.
I: Animals, Bind 10, Nr. 1, 54, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Effect of soybean oil and fish oil on lipid-related transcripts in subcutaneous adipose tissue of dairy cows
AU - Vargas-Bello-Pérez, Einar
AU - Bionaz, Massimo
AU - Garrido-Sartore, Macarena
AU - Cancino-Padilla, Nathaly
AU - Morales, María Sol
AU - Romero, Jaime
AU - Leskinen, Heidi
AU - Garnsworthy, Philip C.
AU - Loor, Juan J.
PY - 2020
Y1 - 2020
N2 - The objective of this study was to determine the effect of long-term supplementation of unsaturated oil on lipid metabolism and transcription of genes involved in lipid metabolism in subcutaneous adipose tissue (SAT) of mid-lactating dairy cows. The objective was achieved by supplementing dairy cows with soybean oil (SO; high in linoleic acid) or fish oil (FO; high in EPA and DHA) for 63 days (nine weeks). Cows were fed a control diet with no added lipid, or diets containing SO or FO (n = 5 cows/group). At the onset of the experiment (day 0) and on days 21, 42, and 63 of supplementation, blood and SAT samples were collected from each animal. Oil supplementation increased cholesterol and NEFA in plasma, with a greater effect of SO compared to FO. Concentration of BUN was lower in SO compared to control and FO at the end of the trial. Transcription of few genes was affected by dietary lipids: FABP4 had lowest expression in FO followed by SO and control. ACACA and FASN had higher expression in FO. Transcription of SCAP was higher but expression of INSIG1 was lower in SO. Overall, results revealed that compared to control, SO and FO had lipogenic effect in SAT.
AB - The objective of this study was to determine the effect of long-term supplementation of unsaturated oil on lipid metabolism and transcription of genes involved in lipid metabolism in subcutaneous adipose tissue (SAT) of mid-lactating dairy cows. The objective was achieved by supplementing dairy cows with soybean oil (SO; high in linoleic acid) or fish oil (FO; high in EPA and DHA) for 63 days (nine weeks). Cows were fed a control diet with no added lipid, or diets containing SO or FO (n = 5 cows/group). At the onset of the experiment (day 0) and on days 21, 42, and 63 of supplementation, blood and SAT samples were collected from each animal. Oil supplementation increased cholesterol and NEFA in plasma, with a greater effect of SO compared to FO. Concentration of BUN was lower in SO compared to control and FO at the end of the trial. Transcription of few genes was affected by dietary lipids: FABP4 had lowest expression in FO followed by SO and control. ACACA and FASN had higher expression in FO. Transcription of SCAP was higher but expression of INSIG1 was lower in SO. Overall, results revealed that compared to control, SO and FO had lipogenic effect in SAT.
KW - Fat supplementation
KW - Lactating cows
KW - Subcutaneous adipose tissue
KW - Transcriptomic
U2 - 10.3390/ani10010054
DO - 10.3390/ani10010054
M3 - Journal article
C2 - 31888021
AN - SCOPUS:85077367307
VL - 10
JO - Animals
JF - Animals
SN - 2076-2615
IS - 1
M1 - 54
ER -
ID: 234210381