Expression of cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method

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Standard

Expression of cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method. / Vlak, Just M.; Schouten, Alexander; Usmany, Magda; Belsham, Graham J.; Klinge-Roode, Els C.; Maule, Andrew J.; Van Lent, Jan W.M.; Zuidema, Douwe.

I: Virology, Bind 179, Nr. 1, 11.1990, s. 312-320.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Vlak, JM, Schouten, A, Usmany, M, Belsham, GJ, Klinge-Roode, EC, Maule, AJ, Van Lent, JWM & Zuidema, D 1990, 'Expression of cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method', Virology, bind 179, nr. 1, s. 312-320. https://doi.org/10.1016/0042-6822(90)90299-7

APA

Vlak, J. M., Schouten, A., Usmany, M., Belsham, G. J., Klinge-Roode, E. C., Maule, A. J., Van Lent, J. W. M., & Zuidema, D. (1990). Expression of cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method. Virology, 179(1), 312-320. https://doi.org/10.1016/0042-6822(90)90299-7

Vancouver

Vlak JM, Schouten A, Usmany M, Belsham GJ, Klinge-Roode EC, Maule AJ o.a. Expression of cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method. Virology. 1990 nov.;179(1):312-320. https://doi.org/10.1016/0042-6822(90)90299-7

Author

Vlak, Just M. ; Schouten, Alexander ; Usmany, Magda ; Belsham, Graham J. ; Klinge-Roode, Els C. ; Maule, Andrew J. ; Van Lent, Jan W.M. ; Zuidema, Douwe. / Expression of cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method. I: Virology. 1990 ; Bind 179, Nr. 1. s. 312-320.

Bibtex

@article{aa012e4d717d4af68cabf9275a44a6dc,
title = "Expression of cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method",
abstract = "A new baculovirus expression vector based upon the pl0 gene of Autographa californica nuclear polyhedrosis virus (AcNPV) and a novel system for the screening of p10 recombinants have been developed. The insertion of a cassette containing the IacZ gene under the control of a heat-shock promoter of Drosophila melanogaster downstream from the cloning site in p10 transfer vectors allows the convenient identification of putative recombinants by virtue of their expression of β-galactosidase. Using this p10 transfer vector an AcNPV recombinant was engineered with a cDNA copy of gene I of cauliflower mosaic virus (CaMV) in place of the p10 coding sequence. This pi 0 recombinant expressed CaMV gene I at levels equivalent to those of p10 and polyhedrin, and was shown to be as effective in producing this protein as recombinants exploiting the polyhedrin promoter. CaMV gene I protein formed large numbers of hollow fiber-like structures in the cytoplasm of infected cells. Because the polyhedrin gene remains intact, these p10 expression vectors may be exploited for the expression of heterologous proteins in insects infected per os and for the enhancement of baculovirus pathogenicity for insect control.",
author = "Vlak, {Just M.} and Alexander Schouten and Magda Usmany and Belsham, {Graham J.} and Klinge-Roode, {Els C.} and Maule, {Andrew J.} and {Van Lent}, {Jan W.M.} and Douwe Zuidema",
note = "Funding Information: We appreciated the expert techntcal assrstance of Joop Groene-wegen In electron microscopy. We acknowledge Dr. 1. Roosien for valuable suggestions and Dr. R. W. Goldbach for his continuous interest. The research of D.Z. is made possrble by a fellowship of the Royal Netherlands Academy of Arts and Sciences. Thus research was supported part by the Bromolecular Action Programme (Contract No. BAP-01 1%NL (Wageningen) and Contract No. BAP-01 19-UK (Pirbright)) of the commission the European Communities.",
year = "1990",
month = nov,
doi = "10.1016/0042-6822(90)90299-7",
language = "English",
volume = "179",
pages = "312--320",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press",
number = "1",

}

RIS

TY - JOUR

T1 - Expression of cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method

AU - Vlak, Just M.

AU - Schouten, Alexander

AU - Usmany, Magda

AU - Belsham, Graham J.

AU - Klinge-Roode, Els C.

AU - Maule, Andrew J.

AU - Van Lent, Jan W.M.

AU - Zuidema, Douwe

N1 - Funding Information: We appreciated the expert techntcal assrstance of Joop Groene-wegen In electron microscopy. We acknowledge Dr. 1. Roosien for valuable suggestions and Dr. R. W. Goldbach for his continuous interest. The research of D.Z. is made possrble by a fellowship of the Royal Netherlands Academy of Arts and Sciences. Thus research was supported part by the Bromolecular Action Programme (Contract No. BAP-01 1%NL (Wageningen) and Contract No. BAP-01 19-UK (Pirbright)) of the commission the European Communities.

PY - 1990/11

Y1 - 1990/11

N2 - A new baculovirus expression vector based upon the pl0 gene of Autographa californica nuclear polyhedrosis virus (AcNPV) and a novel system for the screening of p10 recombinants have been developed. The insertion of a cassette containing the IacZ gene under the control of a heat-shock promoter of Drosophila melanogaster downstream from the cloning site in p10 transfer vectors allows the convenient identification of putative recombinants by virtue of their expression of β-galactosidase. Using this p10 transfer vector an AcNPV recombinant was engineered with a cDNA copy of gene I of cauliflower mosaic virus (CaMV) in place of the p10 coding sequence. This pi 0 recombinant expressed CaMV gene I at levels equivalent to those of p10 and polyhedrin, and was shown to be as effective in producing this protein as recombinants exploiting the polyhedrin promoter. CaMV gene I protein formed large numbers of hollow fiber-like structures in the cytoplasm of infected cells. Because the polyhedrin gene remains intact, these p10 expression vectors may be exploited for the expression of heterologous proteins in insects infected per os and for the enhancement of baculovirus pathogenicity for insect control.

AB - A new baculovirus expression vector based upon the pl0 gene of Autographa californica nuclear polyhedrosis virus (AcNPV) and a novel system for the screening of p10 recombinants have been developed. The insertion of a cassette containing the IacZ gene under the control of a heat-shock promoter of Drosophila melanogaster downstream from the cloning site in p10 transfer vectors allows the convenient identification of putative recombinants by virtue of their expression of β-galactosidase. Using this p10 transfer vector an AcNPV recombinant was engineered with a cDNA copy of gene I of cauliflower mosaic virus (CaMV) in place of the p10 coding sequence. This pi 0 recombinant expressed CaMV gene I at levels equivalent to those of p10 and polyhedrin, and was shown to be as effective in producing this protein as recombinants exploiting the polyhedrin promoter. CaMV gene I protein formed large numbers of hollow fiber-like structures in the cytoplasm of infected cells. Because the polyhedrin gene remains intact, these p10 expression vectors may be exploited for the expression of heterologous proteins in insects infected per os and for the enhancement of baculovirus pathogenicity for insect control.

UR - http://www.scopus.com/inward/record.url?scp=0024997847&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(90)90299-7

DO - 10.1016/0042-6822(90)90299-7

M3 - Journal article

C2 - 2219726

AN - SCOPUS:0024997847

VL - 179

SP - 312

EP - 320

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -

ID: 381228683