Generation of three isogenic gene-edited Huntington’s disease human embryonic stem cell lines with DOX-inducible NGN2 expression cassette in the AAVS1 safe locus

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Standard

Generation of three isogenic gene-edited Huntington’s disease human embryonic stem cell lines with DOX-inducible NGN2 expression cassette in the AAVS1 safe locus. / Duque Villegas, Luisana Carolina; Chandrasekaran, Abinaya; Flintholm Andersen, Sophie Amalie; Nørremølle, Anne; Schmid, Benjamin; Pouladi, Mahmoud A; Freude, Kristine.

I: Stem Cell Research, Bind 77, 103408, 2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Duque Villegas, LC, Chandrasekaran, A, Flintholm Andersen, SA, Nørremølle, A, Schmid, B, Pouladi, MA & Freude, K 2024, 'Generation of three isogenic gene-edited Huntington’s disease human embryonic stem cell lines with DOX-inducible NGN2 expression cassette in the AAVS1 safe locus', Stem Cell Research, bind 77, 103408. https://doi.org/10.1016/j.scr.2024.103408

APA

Duque Villegas, L. C., Chandrasekaran, A., Flintholm Andersen, S. A., Nørremølle, A., Schmid, B., Pouladi, M. A., & Freude, K. (2024). Generation of three isogenic gene-edited Huntington’s disease human embryonic stem cell lines with DOX-inducible NGN2 expression cassette in the AAVS1 safe locus. Stem Cell Research, 77, [103408]. https://doi.org/10.1016/j.scr.2024.103408

Vancouver

Duque Villegas LC, Chandrasekaran A, Flintholm Andersen SA, Nørremølle A, Schmid B, Pouladi MA o.a. Generation of three isogenic gene-edited Huntington’s disease human embryonic stem cell lines with DOX-inducible NGN2 expression cassette in the AAVS1 safe locus. Stem Cell Research. 2024;77. 103408. https://doi.org/10.1016/j.scr.2024.103408

Author

Duque Villegas, Luisana Carolina ; Chandrasekaran, Abinaya ; Flintholm Andersen, Sophie Amalie ; Nørremølle, Anne ; Schmid, Benjamin ; Pouladi, Mahmoud A ; Freude, Kristine. / Generation of three isogenic gene-edited Huntington’s disease human embryonic stem cell lines with DOX-inducible NGN2 expression cassette in the AAVS1 safe locus. I: Stem Cell Research. 2024 ; Bind 77.

Bibtex

@article{72489031eaa0403fb6bc3809f9fce604,
title = "Generation of three isogenic gene-edited Huntington{\textquoteright}s disease human embryonic stem cell lines with DOX-inducible NGN2 expression cassette in the AAVS1 safe locus",
abstract = "Neurogenin 2 (NGN2), a neuronal transcription factor, can expedite differentiation of stem cells into mature glutamatergic neurons. We have utilized an allelic series of previously published and characterized isogenic Huntington's disease (IsoHD) human embryonic stem cell lines (Ooi et al., 2019), carrying different CAG repeat lengths in the first exon of the huntingtin gene. These IsoHDs were modified using CRISPR/Cas9 to insert NGN2 under the TET-ON doxycycline inducible promoter. The resulting IsoHD-NGN2 cell lines retained pluripotency in the absence of doxycycline (DOX), and via addition of DOX to the culturing media differentiation to neurons was achieved within 14 days.",
author = "{Duque Villegas}, {Luisana Carolina} and Abinaya Chandrasekaran and {Flintholm Andersen}, {Sophie Amalie} and Anne N{\o}rrem{\o}lle and Benjamin Schmid and Pouladi, {Mahmoud A} and Kristine Freude",
year = "2024",
doi = "10.1016/j.scr.2024.103408",
language = "English",
volume = "77",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Generation of three isogenic gene-edited Huntington’s disease human embryonic stem cell lines with DOX-inducible NGN2 expression cassette in the AAVS1 safe locus

AU - Duque Villegas, Luisana Carolina

AU - Chandrasekaran, Abinaya

AU - Flintholm Andersen, Sophie Amalie

AU - Nørremølle, Anne

AU - Schmid, Benjamin

AU - Pouladi, Mahmoud A

AU - Freude, Kristine

PY - 2024

Y1 - 2024

N2 - Neurogenin 2 (NGN2), a neuronal transcription factor, can expedite differentiation of stem cells into mature glutamatergic neurons. We have utilized an allelic series of previously published and characterized isogenic Huntington's disease (IsoHD) human embryonic stem cell lines (Ooi et al., 2019), carrying different CAG repeat lengths in the first exon of the huntingtin gene. These IsoHDs were modified using CRISPR/Cas9 to insert NGN2 under the TET-ON doxycycline inducible promoter. The resulting IsoHD-NGN2 cell lines retained pluripotency in the absence of doxycycline (DOX), and via addition of DOX to the culturing media differentiation to neurons was achieved within 14 days.

AB - Neurogenin 2 (NGN2), a neuronal transcription factor, can expedite differentiation of stem cells into mature glutamatergic neurons. We have utilized an allelic series of previously published and characterized isogenic Huntington's disease (IsoHD) human embryonic stem cell lines (Ooi et al., 2019), carrying different CAG repeat lengths in the first exon of the huntingtin gene. These IsoHDs were modified using CRISPR/Cas9 to insert NGN2 under the TET-ON doxycycline inducible promoter. The resulting IsoHD-NGN2 cell lines retained pluripotency in the absence of doxycycline (DOX), and via addition of DOX to the culturing media differentiation to neurons was achieved within 14 days.

U2 - 10.1016/j.scr.2024.103408

DO - 10.1016/j.scr.2024.103408

M3 - Journal article

VL - 77

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

M1 - 103408

ER -

ID: 386778736