Immunization with a vaccinia recombinant expressing the F protein protects Rabbits from challenge with a lethal dose of rinderpest virus

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Standard

Immunization with a vaccinia recombinant expressing the F protein protects Rabbits from challenge with a lethal dose of rinderpest virus. / Barrett, Thomas; Belsham, Graham J.; Subbarao, Shaila M.; Evans, Sharon A.

I: Virology, Bind 170, Nr. 1, 05.1989, s. 11-18.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Barrett, T, Belsham, GJ, Subbarao, SM & Evans, SA 1989, 'Immunization with a vaccinia recombinant expressing the F protein protects Rabbits from challenge with a lethal dose of rinderpest virus', Virology, bind 170, nr. 1, s. 11-18. https://doi.org/10.1016/0042-6822(89)90346-2

APA

Barrett, T., Belsham, G. J., Subbarao, S. M., & Evans, S. A. (1989). Immunization with a vaccinia recombinant expressing the F protein protects Rabbits from challenge with a lethal dose of rinderpest virus. Virology, 170(1), 11-18. https://doi.org/10.1016/0042-6822(89)90346-2

Vancouver

Barrett T, Belsham GJ, Subbarao SM, Evans SA. Immunization with a vaccinia recombinant expressing the F protein protects Rabbits from challenge with a lethal dose of rinderpest virus. Virology. 1989 maj;170(1):11-18. https://doi.org/10.1016/0042-6822(89)90346-2

Author

Barrett, Thomas ; Belsham, Graham J. ; Subbarao, Shaila M. ; Evans, Sharon A. / Immunization with a vaccinia recombinant expressing the F protein protects Rabbits from challenge with a lethal dose of rinderpest virus. I: Virology. 1989 ; Bind 170, Nr. 1. s. 11-18.

Bibtex

@article{bf74ad865c1b41a3937063014d15863c,
title = "Immunization with a vaccinia recombinant expressing the F protein protects Rabbits from challenge with a lethal dose of rinderpest virus",
abstract = "A cDNA clone containing the complete coding sequence of the rinderpest fusion protein (F) gene was inserted into the thymidine kinase gene of vaccinia virus (WR strain) under the control of the 7.5K early/late vaccinia virus promoter. All forms of the F protein, i.e., the glycosylated Fo precursor, the unglycosylated F1 protein, and the glycosylated F2 protein, were detected in cells infected with the recombinant virus. Vaccination of rabbits with the recombinant virus induced antibodies which reacted in an ELISA system specific for rinderpest. The rabbit sera contained neutralizing antibodies against rinderpest virus and precipitated the F protein from lysates of rinderpest infected cells. Rabbits vaccinated with the recombinant rinderpest F gene vaccinia virus were protected from a lethal challenge with the lapinized Nakamura 3 strain of rinderpest virus. Variations in the severity of clinical symptoms correlated with the level of anti-F protein antibodies produced.",
author = "Thomas Barrett and Belsham, {Graham J.} and Subbarao, {Shaila M.} and Evans, {Sharon A.}",
note = "Funding Information: We thank Drs. B. W. J. Mahyand C. Bostock for helpful discussions and critical reading of the manuscript, Miss Julia Brangwyn and Miss Lynette Goatley for excellent technical assistance, and Mr. Len Pullen for carrying out the animal experimental work. This work was supported by a grant from the Wellcome Trust (No. 14294/1.5). Dr. S. M. Subbarao was the recipient of a Rockefeller Foundation Biotechnology Career Fellowship.",
year = "1989",
month = may,
doi = "10.1016/0042-6822(89)90346-2",
language = "English",
volume = "170",
pages = "11--18",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press",
number = "1",

}

RIS

TY - JOUR

T1 - Immunization with a vaccinia recombinant expressing the F protein protects Rabbits from challenge with a lethal dose of rinderpest virus

AU - Barrett, Thomas

AU - Belsham, Graham J.

AU - Subbarao, Shaila M.

AU - Evans, Sharon A.

N1 - Funding Information: We thank Drs. B. W. J. Mahyand C. Bostock for helpful discussions and critical reading of the manuscript, Miss Julia Brangwyn and Miss Lynette Goatley for excellent technical assistance, and Mr. Len Pullen for carrying out the animal experimental work. This work was supported by a grant from the Wellcome Trust (No. 14294/1.5). Dr. S. M. Subbarao was the recipient of a Rockefeller Foundation Biotechnology Career Fellowship.

PY - 1989/5

Y1 - 1989/5

N2 - A cDNA clone containing the complete coding sequence of the rinderpest fusion protein (F) gene was inserted into the thymidine kinase gene of vaccinia virus (WR strain) under the control of the 7.5K early/late vaccinia virus promoter. All forms of the F protein, i.e., the glycosylated Fo precursor, the unglycosylated F1 protein, and the glycosylated F2 protein, were detected in cells infected with the recombinant virus. Vaccination of rabbits with the recombinant virus induced antibodies which reacted in an ELISA system specific for rinderpest. The rabbit sera contained neutralizing antibodies against rinderpest virus and precipitated the F protein from lysates of rinderpest infected cells. Rabbits vaccinated with the recombinant rinderpest F gene vaccinia virus were protected from a lethal challenge with the lapinized Nakamura 3 strain of rinderpest virus. Variations in the severity of clinical symptoms correlated with the level of anti-F protein antibodies produced.

AB - A cDNA clone containing the complete coding sequence of the rinderpest fusion protein (F) gene was inserted into the thymidine kinase gene of vaccinia virus (WR strain) under the control of the 7.5K early/late vaccinia virus promoter. All forms of the F protein, i.e., the glycosylated Fo precursor, the unglycosylated F1 protein, and the glycosylated F2 protein, were detected in cells infected with the recombinant virus. Vaccination of rabbits with the recombinant virus induced antibodies which reacted in an ELISA system specific for rinderpest. The rabbit sera contained neutralizing antibodies against rinderpest virus and precipitated the F protein from lysates of rinderpest infected cells. Rabbits vaccinated with the recombinant rinderpest F gene vaccinia virus were protected from a lethal challenge with the lapinized Nakamura 3 strain of rinderpest virus. Variations in the severity of clinical symptoms correlated with the level of anti-F protein antibodies produced.

UR - http://www.scopus.com/inward/record.url?scp=0024559770&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(89)90346-2

DO - 10.1016/0042-6822(89)90346-2

M3 - Journal article

C2 - 2718375

AN - SCOPUS:0024559770

VL - 170

SP - 11

EP - 18

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -

ID: 381228532