Ribotypes and plasmid contents of Vibrio anguillarum strains in relation to serovar
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Ribotypes and plasmid contents of Vibrio anguillarum strains in relation to serovar. / Olsen, J. E.; Larsen, J. L.
I: Applied and Environmental Microbiology, Bind 59, Nr. 11, 1993, s. 3863-3870.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Ribotypes and plasmid contents of Vibrio anguillarum strains in relation to serovar
AU - Olsen, J. E.
AU - Larsen, J. L.
PY - 1993
Y1 - 1993
N2 - Eighty-six strains of the 10 major agglutination types of Vibrio anguillarum (serovars O1 to O10) and 6 nontypeable strains of V. anguillarum have been characterized by ribotyping with a probe complementary to 16S and 23S rRNA of Escherichia coli and by plasmid profile analysis. Forty-four different ribotypes were observed with the restriction enzyme HindIII. Ribotype similarity was compared by using the Dice coefficient (S(d)), and three significantly different levels of homogeneity within the V. anguillarum serovars were observed (serovars O1, O3A, O7, and O9, S(d)s of >90%; serovars O2B, O4, and O10, S(d)s of 80 to 90%; serovars O2A, O3B, O5, and O8, S(d)s between 46 and 70%). None of the ribotype patterns of V. anguillarum strains were observed among 20 other Vibrio strains typed for comparison. By cluster analysis, the V. anguillarum strains were divided into a main cluster containing 83 strains, while all strains of serovar O3B, one strain (each) of serovars O2A, O5, and O8, and a nontypeable strain were separated from this cluster by at least 15% difference in similarity coefficients. Plasmids were demonstrated in only six strains other than serovar O1. In serovar O1, a 67- to 70-kilobase-pair (kb) plasmid molecule was present in 17 of 19 strains tested; of the two remaining strains, one strain harbored two plasmids, (45 and 6.5 kb) and one strain had no plasmids.
AB - Eighty-six strains of the 10 major agglutination types of Vibrio anguillarum (serovars O1 to O10) and 6 nontypeable strains of V. anguillarum have been characterized by ribotyping with a probe complementary to 16S and 23S rRNA of Escherichia coli and by plasmid profile analysis. Forty-four different ribotypes were observed with the restriction enzyme HindIII. Ribotype similarity was compared by using the Dice coefficient (S(d)), and three significantly different levels of homogeneity within the V. anguillarum serovars were observed (serovars O1, O3A, O7, and O9, S(d)s of >90%; serovars O2B, O4, and O10, S(d)s of 80 to 90%; serovars O2A, O3B, O5, and O8, S(d)s between 46 and 70%). None of the ribotype patterns of V. anguillarum strains were observed among 20 other Vibrio strains typed for comparison. By cluster analysis, the V. anguillarum strains were divided into a main cluster containing 83 strains, while all strains of serovar O3B, one strain (each) of serovars O2A, O5, and O8, and a nontypeable strain were separated from this cluster by at least 15% difference in similarity coefficients. Plasmids were demonstrated in only six strains other than serovar O1. In serovar O1, a 67- to 70-kilobase-pair (kb) plasmid molecule was present in 17 of 19 strains tested; of the two remaining strains, one strain harbored two plasmids, (45 and 6.5 kb) and one strain had no plasmids.
U2 - 10.1128/aem.59.11.3863-3870.1993
DO - 10.1128/aem.59.11.3863-3870.1993
M3 - Journal article
C2 - 7506897
AN - SCOPUS:0027453387
VL - 59
SP - 3863
EP - 3870
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 11
ER -
ID: 251186761