(R)-α-Lipoic acid-supplemented old rats have improved mitochondrial function, decreased oxidative damage, and increased metabolic rate

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Standard

(R)-α-Lipoic acid-supplemented old rats have improved mitochondrial function, decreased oxidative damage, and increased metabolic rate. / Hagen, Tory M.; Ingersoll, Russell T.; Lykkesfeldt, Jens; Liu, Jiankang; Wehr, Carol M.; Vinarsky, Vladimir; Bartholomew, James C.; Ames, Bruce N.

I: FASEB Journal, Bind 13, Nr. 2, 16.02.1999, s. 411-418.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Hagen, TM, Ingersoll, RT, Lykkesfeldt, J, Liu, J, Wehr, CM, Vinarsky, V, Bartholomew, JC & Ames, BN 1999, '(R)-α-Lipoic acid-supplemented old rats have improved mitochondrial function, decreased oxidative damage, and increased metabolic rate', FASEB Journal, bind 13, nr. 2, s. 411-418.

APA

Hagen, T. M., Ingersoll, R. T., Lykkesfeldt, J., Liu, J., Wehr, C. M., Vinarsky, V., Bartholomew, J. C., & Ames, B. N. (1999). (R)-α-Lipoic acid-supplemented old rats have improved mitochondrial function, decreased oxidative damage, and increased metabolic rate. FASEB Journal, 13(2), 411-418.

Vancouver

Hagen TM, Ingersoll RT, Lykkesfeldt J, Liu J, Wehr CM, Vinarsky V o.a. (R)-α-Lipoic acid-supplemented old rats have improved mitochondrial function, decreased oxidative damage, and increased metabolic rate. FASEB Journal. 1999 feb. 16;13(2):411-418.

Author

Hagen, Tory M. ; Ingersoll, Russell T. ; Lykkesfeldt, Jens ; Liu, Jiankang ; Wehr, Carol M. ; Vinarsky, Vladimir ; Bartholomew, James C. ; Ames, Bruce N. / (R)-α-Lipoic acid-supplemented old rats have improved mitochondrial function, decreased oxidative damage, and increased metabolic rate. I: FASEB Journal. 1999 ; Bind 13, Nr. 2. s. 411-418.

Bibtex

@article{b6ccc0e9f9af488dae20f1a740435539,
title = "(R)-α-Lipoic acid-supplemented old rats have improved mitochondrial function, decreased oxidative damage, and increased metabolic rate",
abstract = "A diet supplemented with (R)-lipoic acid, a mitochondrial coenzyme, was fed to old rats to determine its efficacy in reversing the decline in metabolism seen with age. Young (3 to 5 months) and old (24 to 26 months) rats were fed an AIN-93M diet with or without (R)-lipoic acid (0.5% w/w) for 2 wk, killed, and their liver parenchymal cells were isolated. Hepatocytes from untreated old rats vs. young controls had significantly lower oxygen consumption (P<0.03) and mitochondrial membrane potential. (R)-Lipoic acid supplementation reversed the age-related decline in O2 consumption and increased (P<0.03) mitochondrial membrane potential. Ambulatory activity, a measure of general metabolic activity, was almost threefold lower in untreated old rats vs. controls, but this decline was reversed (P<0.005) in old rats fed (R)-lipoic acid. The increase of oxidants with age, as measured by the fluorescence produced on oxidizing 2',7'-dichlorofluorescin, was significantly lowered in (R)-lipoic acid supplemented old rats (P< 0.01). Malondialdehyde (MBA) levels, an indicator of lipid peroxidation, were increased fivefold with age in cells from unsupplemented rats. Feeding rats the (R)-lipoic acid diet reduced MDA levels markedly (P<0.01). Both glutathione and ascorbic acid levels declined in hepatocytes with age, but their loss was completely reversed with (R)-lipoic acid supplementation. Thus, (R)-lipoic acid supplementation improves indices of metabolic activity as well as lowers oxidative stress and damage evident in aging.",
keywords = "Aging, Ambulatory activity, Antioxidants, Liver, MDA",
author = "Hagen, {Tory M.} and Ingersoll, {Russell T.} and Jens Lykkesfeldt and Jiankang Liu and Wehr, {Carol M.} and Vladimir Vinarsky and Bartholomew, {James C.} and Ames, {Bruce N.}",
year = "1999",
month = feb,
day = "16",
language = "English",
volume = "13",
pages = "411--418",
journal = "F A S E B Journal",
issn = "0892-6638",
publisher = "Federation of American Societies for Experimental Biology",
number = "2",

}

RIS

TY - JOUR

T1 - (R)-α-Lipoic acid-supplemented old rats have improved mitochondrial function, decreased oxidative damage, and increased metabolic rate

AU - Hagen, Tory M.

AU - Ingersoll, Russell T.

AU - Lykkesfeldt, Jens

AU - Liu, Jiankang

AU - Wehr, Carol M.

AU - Vinarsky, Vladimir

AU - Bartholomew, James C.

AU - Ames, Bruce N.

PY - 1999/2/16

Y1 - 1999/2/16

N2 - A diet supplemented with (R)-lipoic acid, a mitochondrial coenzyme, was fed to old rats to determine its efficacy in reversing the decline in metabolism seen with age. Young (3 to 5 months) and old (24 to 26 months) rats were fed an AIN-93M diet with or without (R)-lipoic acid (0.5% w/w) for 2 wk, killed, and their liver parenchymal cells were isolated. Hepatocytes from untreated old rats vs. young controls had significantly lower oxygen consumption (P<0.03) and mitochondrial membrane potential. (R)-Lipoic acid supplementation reversed the age-related decline in O2 consumption and increased (P<0.03) mitochondrial membrane potential. Ambulatory activity, a measure of general metabolic activity, was almost threefold lower in untreated old rats vs. controls, but this decline was reversed (P<0.005) in old rats fed (R)-lipoic acid. The increase of oxidants with age, as measured by the fluorescence produced on oxidizing 2',7'-dichlorofluorescin, was significantly lowered in (R)-lipoic acid supplemented old rats (P< 0.01). Malondialdehyde (MBA) levels, an indicator of lipid peroxidation, were increased fivefold with age in cells from unsupplemented rats. Feeding rats the (R)-lipoic acid diet reduced MDA levels markedly (P<0.01). Both glutathione and ascorbic acid levels declined in hepatocytes with age, but their loss was completely reversed with (R)-lipoic acid supplementation. Thus, (R)-lipoic acid supplementation improves indices of metabolic activity as well as lowers oxidative stress and damage evident in aging.

AB - A diet supplemented with (R)-lipoic acid, a mitochondrial coenzyme, was fed to old rats to determine its efficacy in reversing the decline in metabolism seen with age. Young (3 to 5 months) and old (24 to 26 months) rats were fed an AIN-93M diet with or without (R)-lipoic acid (0.5% w/w) for 2 wk, killed, and their liver parenchymal cells were isolated. Hepatocytes from untreated old rats vs. young controls had significantly lower oxygen consumption (P<0.03) and mitochondrial membrane potential. (R)-Lipoic acid supplementation reversed the age-related decline in O2 consumption and increased (P<0.03) mitochondrial membrane potential. Ambulatory activity, a measure of general metabolic activity, was almost threefold lower in untreated old rats vs. controls, but this decline was reversed (P<0.005) in old rats fed (R)-lipoic acid. The increase of oxidants with age, as measured by the fluorescence produced on oxidizing 2',7'-dichlorofluorescin, was significantly lowered in (R)-lipoic acid supplemented old rats (P< 0.01). Malondialdehyde (MBA) levels, an indicator of lipid peroxidation, were increased fivefold with age in cells from unsupplemented rats. Feeding rats the (R)-lipoic acid diet reduced MDA levels markedly (P<0.01). Both glutathione and ascorbic acid levels declined in hepatocytes with age, but their loss was completely reversed with (R)-lipoic acid supplementation. Thus, (R)-lipoic acid supplementation improves indices of metabolic activity as well as lowers oxidative stress and damage evident in aging.

KW - Aging

KW - Ambulatory activity

KW - Antioxidants

KW - Liver

KW - MDA

UR - http://www.scopus.com/inward/record.url?scp=0033042929&partnerID=8YFLogxK

M3 - Journal article

C2 - 9973329

AN - SCOPUS:0033042929

VL - 13

SP - 411

EP - 418

JO - F A S E B Journal

JF - F A S E B Journal

SN - 0892-6638

IS - 2

ER -

ID: 204500310