Studies on the infectivity of foot-and-mouth disease virus RNA using microinjection

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Standard

Studies on the infectivity of foot-and-mouth disease virus RNA using microinjection. / Belsham, G. J.; Bostock, C. J.

I: Journal of General Virology, Bind 69, Nr. 2, 1988, s. 265-274.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Belsham, GJ & Bostock, CJ 1988, 'Studies on the infectivity of foot-and-mouth disease virus RNA using microinjection', Journal of General Virology, bind 69, nr. 2, s. 265-274. https://doi.org/10.1099/0022-1317-69-2-265

APA

Belsham, G. J., & Bostock, C. J. (1988). Studies on the infectivity of foot-and-mouth disease virus RNA using microinjection. Journal of General Virology, 69(2), 265-274. https://doi.org/10.1099/0022-1317-69-2-265

Vancouver

Belsham GJ, Bostock CJ. Studies on the infectivity of foot-and-mouth disease virus RNA using microinjection. Journal of General Virology. 1988;69(2):265-274. https://doi.org/10.1099/0022-1317-69-2-265

Author

Belsham, G. J. ; Bostock, C. J. / Studies on the infectivity of foot-and-mouth disease virus RNA using microinjection. I: Journal of General Virology. 1988 ; Bind 69, Nr. 2. s. 265-274.

Bibtex

@article{bedfa77d31fe4464a66894cc05113eb0,
title = "Studies on the infectivity of foot-and-mouth disease virus RNA using microinjection",
abstract = "Foot-and-mouth disease virus (FMDV) RNA, isolated as virion RNA from purified virus particles or as total RNA from infected cells, has been microinjected into nuclei and cytoplasms of BHK cells. When injected directly into the nucleus FMDV RNA was not infectious, whereas cytoplasmic injection resulted in a high proportion of productive infections. Infectivity microinjection assays on dilution series of various FMDV RNAs showed that both single-stranded positive sense 35S RNA and double-stranded replicative form (Rf) RNA have an infectivity close to 1 p.f.u. per molecule, although only a minor fraction of BHK cells appeared able to support plaque formation following microinjection of Rf FMDV RNA. The infectivity of Rf FMDV RNA was not sensitive to inhibition by actinomycin D. The results are discussed in relation to the high virus particle to p.f.u. ratios observed for FMDV.",
author = "Belsham, {G. J.} and Bostock, {C. J.}",
year = "1988",
doi = "10.1099/0022-1317-69-2-265",
language = "English",
volume = "69",
pages = "265--274",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "2",

}

RIS

TY - JOUR

T1 - Studies on the infectivity of foot-and-mouth disease virus RNA using microinjection

AU - Belsham, G. J.

AU - Bostock, C. J.

PY - 1988

Y1 - 1988

N2 - Foot-and-mouth disease virus (FMDV) RNA, isolated as virion RNA from purified virus particles or as total RNA from infected cells, has been microinjected into nuclei and cytoplasms of BHK cells. When injected directly into the nucleus FMDV RNA was not infectious, whereas cytoplasmic injection resulted in a high proportion of productive infections. Infectivity microinjection assays on dilution series of various FMDV RNAs showed that both single-stranded positive sense 35S RNA and double-stranded replicative form (Rf) RNA have an infectivity close to 1 p.f.u. per molecule, although only a minor fraction of BHK cells appeared able to support plaque formation following microinjection of Rf FMDV RNA. The infectivity of Rf FMDV RNA was not sensitive to inhibition by actinomycin D. The results are discussed in relation to the high virus particle to p.f.u. ratios observed for FMDV.

AB - Foot-and-mouth disease virus (FMDV) RNA, isolated as virion RNA from purified virus particles or as total RNA from infected cells, has been microinjected into nuclei and cytoplasms of BHK cells. When injected directly into the nucleus FMDV RNA was not infectious, whereas cytoplasmic injection resulted in a high proportion of productive infections. Infectivity microinjection assays on dilution series of various FMDV RNAs showed that both single-stranded positive sense 35S RNA and double-stranded replicative form (Rf) RNA have an infectivity close to 1 p.f.u. per molecule, although only a minor fraction of BHK cells appeared able to support plaque formation following microinjection of Rf FMDV RNA. The infectivity of Rf FMDV RNA was not sensitive to inhibition by actinomycin D. The results are discussed in relation to the high virus particle to p.f.u. ratios observed for FMDV.

UR - http://www.scopus.com/inward/record.url?scp=0023860172&partnerID=8YFLogxK

U2 - 10.1099/0022-1317-69-2-265

DO - 10.1099/0022-1317-69-2-265

M3 - Journal article

C2 - 2448416

AN - SCOPUS:0023860172

VL - 69

SP - 265

EP - 274

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 2

ER -

ID: 381229014