Synthesis of benzylglucosinolate in Tropaeolum majus L. Isothiocyanates as potent enzyme inhibitors

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Standard

Synthesis of benzylglucosinolate in Tropaeolum majus L. Isothiocyanates as potent enzyme inhibitors. / Lykkesfeldt, Jens; Møller, Birger Lindberg.

I: Plant Physiology, Bind 102, Nr. 2, 01.01.1993, s. 609-613.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Lykkesfeldt, J & Møller, BL 1993, 'Synthesis of benzylglucosinolate in Tropaeolum majus L. Isothiocyanates as potent enzyme inhibitors', Plant Physiology, bind 102, nr. 2, s. 609-613. https://doi.org/10.1104/pp.102.2.609

APA

Lykkesfeldt, J., & Møller, B. L. (1993). Synthesis of benzylglucosinolate in Tropaeolum majus L. Isothiocyanates as potent enzyme inhibitors. Plant Physiology, 102(2), 609-613. https://doi.org/10.1104/pp.102.2.609

Vancouver

Lykkesfeldt J, Møller BL. Synthesis of benzylglucosinolate in Tropaeolum majus L. Isothiocyanates as potent enzyme inhibitors. Plant Physiology. 1993 jan. 1;102(2):609-613. https://doi.org/10.1104/pp.102.2.609

Author

Lykkesfeldt, Jens ; Møller, Birger Lindberg. / Synthesis of benzylglucosinolate in Tropaeolum majus L. Isothiocyanates as potent enzyme inhibitors. I: Plant Physiology. 1993 ; Bind 102, Nr. 2. s. 609-613.

Bibtex

@article{35d1d299dca843669b5afef5af5f4c0e,
title = "Synthesis of benzylglucosinolate in Tropaeolum majus L.: Isothiocyanates as potent enzyme inhibitors",
abstract = "Benzylglucosinolate accumulates in mature plants of Tropaeolum majus L. The biosynthetic capacity for synthesis of benzylglucosinolate and the total content of benzylglucosinolate have been investigated during plant development and in different tissues. The content increased from 5 mg of benzylglucosinolate in the fresh seed to between 200 and 400 mg in the adult plant, depending on size. The biosynthetic capacity was measured using L-[U-14C]phenylalanine as precursor. Incorporation levels of approximately 30% were obtained with green leaves, whereas the incorporation levels obtained with other tissues were in the range of 0 to 5%. Leaves were the primary site of benzylglucosinolate synthesis. The high amounts of benzylglucosinolate accumulated in other tissues (e.g. developing seeds) reflected transport of benzylglucosinolate from the leaves. The initial steps in the biosynthesis of glucosinolates and cyanogenic glycosides are thought to be similar and to be localized on microsomal membranes. However, a microsomal system prepared from T. majus was biosynthetically inactive. Inclusion of T. mijus plant material during preparation of sorghum microsomes also inhibited their activity. Benzylisothiocyanate, generated by degradation of benzylglucosinolate during the homogenization procedure, strongly inhibited the sorghum enzyme system, and its presence may thus explain why the isolated T. majus microsomal system is inactive.",
author = "Jens Lykkesfeldt and M{\o}ller, {Birger Lindberg}",
year = "1993",
month = jan,
day = "1",
doi = "10.1104/pp.102.2.609",
language = "English",
volume = "102",
pages = "609--613",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "2",

}

RIS

TY - JOUR

T1 - Synthesis of benzylglucosinolate in Tropaeolum majus L.

T2 - Isothiocyanates as potent enzyme inhibitors

AU - Lykkesfeldt, Jens

AU - Møller, Birger Lindberg

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Benzylglucosinolate accumulates in mature plants of Tropaeolum majus L. The biosynthetic capacity for synthesis of benzylglucosinolate and the total content of benzylglucosinolate have been investigated during plant development and in different tissues. The content increased from 5 mg of benzylglucosinolate in the fresh seed to between 200 and 400 mg in the adult plant, depending on size. The biosynthetic capacity was measured using L-[U-14C]phenylalanine as precursor. Incorporation levels of approximately 30% were obtained with green leaves, whereas the incorporation levels obtained with other tissues were in the range of 0 to 5%. Leaves were the primary site of benzylglucosinolate synthesis. The high amounts of benzylglucosinolate accumulated in other tissues (e.g. developing seeds) reflected transport of benzylglucosinolate from the leaves. The initial steps in the biosynthesis of glucosinolates and cyanogenic glycosides are thought to be similar and to be localized on microsomal membranes. However, a microsomal system prepared from T. majus was biosynthetically inactive. Inclusion of T. mijus plant material during preparation of sorghum microsomes also inhibited their activity. Benzylisothiocyanate, generated by degradation of benzylglucosinolate during the homogenization procedure, strongly inhibited the sorghum enzyme system, and its presence may thus explain why the isolated T. majus microsomal system is inactive.

AB - Benzylglucosinolate accumulates in mature plants of Tropaeolum majus L. The biosynthetic capacity for synthesis of benzylglucosinolate and the total content of benzylglucosinolate have been investigated during plant development and in different tissues. The content increased from 5 mg of benzylglucosinolate in the fresh seed to between 200 and 400 mg in the adult plant, depending on size. The biosynthetic capacity was measured using L-[U-14C]phenylalanine as precursor. Incorporation levels of approximately 30% were obtained with green leaves, whereas the incorporation levels obtained with other tissues were in the range of 0 to 5%. Leaves were the primary site of benzylglucosinolate synthesis. The high amounts of benzylglucosinolate accumulated in other tissues (e.g. developing seeds) reflected transport of benzylglucosinolate from the leaves. The initial steps in the biosynthesis of glucosinolates and cyanogenic glycosides are thought to be similar and to be localized on microsomal membranes. However, a microsomal system prepared from T. majus was biosynthetically inactive. Inclusion of T. mijus plant material during preparation of sorghum microsomes also inhibited their activity. Benzylisothiocyanate, generated by degradation of benzylglucosinolate during the homogenization procedure, strongly inhibited the sorghum enzyme system, and its presence may thus explain why the isolated T. majus microsomal system is inactive.

U2 - 10.1104/pp.102.2.609

DO - 10.1104/pp.102.2.609

M3 - Journal article

AN - SCOPUS:0001669361

VL - 102

SP - 609

EP - 613

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 2

ER -

ID: 204499583