Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

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Standard

Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response. / Bru, Jean-Louis; Siryaporn, Albert; Høyland-Kroghsbo, Nina Molin.

I: Journal of visualized experiments : JoVE, Nr. 159, e60915, 2020.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Bru, J-L, Siryaporn, A & Høyland-Kroghsbo, NM 2020, 'Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response', Journal of visualized experiments : JoVE, nr. 159, e60915. https://doi.org/10.3791/60915

APA

Bru, J-L., Siryaporn, A., & Høyland-Kroghsbo, N. M. (2020). Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response. Journal of visualized experiments : JoVE, (159), [e60915]. https://doi.org/10.3791/60915

Vancouver

Bru J-L, Siryaporn A, Høyland-Kroghsbo NM. Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response. Journal of visualized experiments : JoVE. 2020;(159). e60915. https://doi.org/10.3791/60915

Author

Bru, Jean-Louis ; Siryaporn, Albert ; Høyland-Kroghsbo, Nina Molin. / Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response. I: Journal of visualized experiments : JoVE. 2020 ; Nr. 159.

Bibtex

@article{8515432fd9e54afb892357ff42a71a14,
title = "Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response",
abstract = "Swarming is a form of surface motility observed in many bacterial species including Pseudomonas aeruginosa and Escherichia coli. Here, dense populations of bacteria move over large distances in characteristic tendril-shaped communities over the course of hours. Swarming is sensitive to several factors including medium moisture, humidity, and nutrient content. In addition, the collective stress response, which is observed in P. aeruginosa that are stressed by antibiotics or bacteriophage (phage), repels swarms from approaching the area containing the stress. The methods described here address how to control the critical factors that affect swarming. We introduce a simple method to monitor swarming dynamics and the collective stress response with high temporal resolution using a flatbed document scanner, and describe how to compile and perform a quantitative analysis of swarms. This simple and cost-effective method provides precise and well-controlled quantification of swarming and may be extended to other types of plate-based growth assays and bacterial species.",
author = "Jean-Louis Bru and Albert Siryaporn and H{\o}yland-Kroghsbo, {Nina Molin}",
year = "2020",
doi = "10.3791/60915",
language = "English",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "Journal of Visualized Experiments",
number = "159",

}

RIS

TY - JOUR

T1 - Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

AU - Bru, Jean-Louis

AU - Siryaporn, Albert

AU - Høyland-Kroghsbo, Nina Molin

PY - 2020

Y1 - 2020

N2 - Swarming is a form of surface motility observed in many bacterial species including Pseudomonas aeruginosa and Escherichia coli. Here, dense populations of bacteria move over large distances in characteristic tendril-shaped communities over the course of hours. Swarming is sensitive to several factors including medium moisture, humidity, and nutrient content. In addition, the collective stress response, which is observed in P. aeruginosa that are stressed by antibiotics or bacteriophage (phage), repels swarms from approaching the area containing the stress. The methods described here address how to control the critical factors that affect swarming. We introduce a simple method to monitor swarming dynamics and the collective stress response with high temporal resolution using a flatbed document scanner, and describe how to compile and perform a quantitative analysis of swarms. This simple and cost-effective method provides precise and well-controlled quantification of swarming and may be extended to other types of plate-based growth assays and bacterial species.

AB - Swarming is a form of surface motility observed in many bacterial species including Pseudomonas aeruginosa and Escherichia coli. Here, dense populations of bacteria move over large distances in characteristic tendril-shaped communities over the course of hours. Swarming is sensitive to several factors including medium moisture, humidity, and nutrient content. In addition, the collective stress response, which is observed in P. aeruginosa that are stressed by antibiotics or bacteriophage (phage), repels swarms from approaching the area containing the stress. The methods described here address how to control the critical factors that affect swarming. We introduce a simple method to monitor swarming dynamics and the collective stress response with high temporal resolution using a flatbed document scanner, and describe how to compile and perform a quantitative analysis of swarms. This simple and cost-effective method provides precise and well-controlled quantification of swarming and may be extended to other types of plate-based growth assays and bacterial species.

U2 - 10.3791/60915

DO - 10.3791/60915

M3 - Journal article

C2 - 32510504

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 159

M1 - e60915

ER -

ID: 243061065