Identification of cross-reacting T-cell epitopes in structural and non-structural proteins of swine and pandemic H1N1 influenza a virus strains in pigs

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Identification of cross-reacting T-cell epitopes in structural and non-structural proteins of swine and pandemic H1N1 influenza a virus strains in pigs. / Baratelli, Massimiliano; Pedersen, Lasse Eggers; Trebbien, Ramona; Larsen, Lars Erik; Jungersen, Gregers; Blanco, Esther; Nielsen, Jens; Montoya, Maria.

I: Journal of General Virology, Bind 98, Nr. 5, 000748, 2017, s. 895-899.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Baratelli, M, Pedersen, LE, Trebbien, R, Larsen, LE, Jungersen, G, Blanco, E, Nielsen, J & Montoya, M 2017, 'Identification of cross-reacting T-cell epitopes in structural and non-structural proteins of swine and pandemic H1N1 influenza a virus strains in pigs', Journal of General Virology, bind 98, nr. 5, 000748, s. 895-899. https://doi.org/10.1099/jgv.0.000748

APA

Baratelli, M., Pedersen, L. E., Trebbien, R., Larsen, L. E., Jungersen, G., Blanco, E., Nielsen, J., & Montoya, M. (2017). Identification of cross-reacting T-cell epitopes in structural and non-structural proteins of swine and pandemic H1N1 influenza a virus strains in pigs. Journal of General Virology, 98(5), 895-899. [000748]. https://doi.org/10.1099/jgv.0.000748

Vancouver

Baratelli M, Pedersen LE, Trebbien R, Larsen LE, Jungersen G, Blanco E o.a. Identification of cross-reacting T-cell epitopes in structural and non-structural proteins of swine and pandemic H1N1 influenza a virus strains in pigs. Journal of General Virology. 2017;98(5):895-899. 000748. https://doi.org/10.1099/jgv.0.000748

Author

Baratelli, Massimiliano ; Pedersen, Lasse Eggers ; Trebbien, Ramona ; Larsen, Lars Erik ; Jungersen, Gregers ; Blanco, Esther ; Nielsen, Jens ; Montoya, Maria. / Identification of cross-reacting T-cell epitopes in structural and non-structural proteins of swine and pandemic H1N1 influenza a virus strains in pigs. I: Journal of General Virology. 2017 ; Bind 98, Nr. 5. s. 895-899.

Bibtex

@article{96c801c2942b4915a20370c2a9838463,
title = "Identification of cross-reacting T-cell epitopes in structural and non-structural proteins of swine and pandemic H1N1 influenza a virus strains in pigs",
abstract = "Heterologous protection against swine influenza viruses (SwIVs) of different lineages is an important concern for the pig industry. Cross-protection between {\textquoteleft}avian-like{\textquoteright} H1N1 and 2009 pandemic H1N1 lineages has been observed previously, indicating the involvement of cross-reacting T-cells. Here, reverse vaccinology was applied to identify cross-reacting MHC class I T-cell epitopes from two different SwIV H1 lineages in pigs. In silico prediction followed by in vitro and in vivo testing was used to identify SLA-1*0702 T-cell epitopes in heterologous SwIV-infected pigs. Following viral infection, tetramer specific T-cell populations were identified. The majority of the identified T-cell epitopes were conserved between the examined lineages, suggesting that targeting cross-reactive T-cell epitopes could be used to improve vaccines against SwIV in SLA-1*0702-positive pigs.",
keywords = "Cross-reactive epitopes, MHC class I T cell epitopes, Reverse vaccinology, Swine influenza virus",
author = "Massimiliano Baratelli and Pedersen, {Lasse Eggers} and Ramona Trebbien and Larsen, {Lars Erik} and Gregers Jungersen and Esther Blanco and Jens Nielsen and Maria Montoya",
year = "2017",
doi = "10.1099/jgv.0.000748",
language = "English",
volume = "98",
pages = "895--899",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "5",

}

RIS

TY - JOUR

T1 - Identification of cross-reacting T-cell epitopes in structural and non-structural proteins of swine and pandemic H1N1 influenza a virus strains in pigs

AU - Baratelli, Massimiliano

AU - Pedersen, Lasse Eggers

AU - Trebbien, Ramona

AU - Larsen, Lars Erik

AU - Jungersen, Gregers

AU - Blanco, Esther

AU - Nielsen, Jens

AU - Montoya, Maria

PY - 2017

Y1 - 2017

N2 - Heterologous protection against swine influenza viruses (SwIVs) of different lineages is an important concern for the pig industry. Cross-protection between ‘avian-like’ H1N1 and 2009 pandemic H1N1 lineages has been observed previously, indicating the involvement of cross-reacting T-cells. Here, reverse vaccinology was applied to identify cross-reacting MHC class I T-cell epitopes from two different SwIV H1 lineages in pigs. In silico prediction followed by in vitro and in vivo testing was used to identify SLA-1*0702 T-cell epitopes in heterologous SwIV-infected pigs. Following viral infection, tetramer specific T-cell populations were identified. The majority of the identified T-cell epitopes were conserved between the examined lineages, suggesting that targeting cross-reactive T-cell epitopes could be used to improve vaccines against SwIV in SLA-1*0702-positive pigs.

AB - Heterologous protection against swine influenza viruses (SwIVs) of different lineages is an important concern for the pig industry. Cross-protection between ‘avian-like’ H1N1 and 2009 pandemic H1N1 lineages has been observed previously, indicating the involvement of cross-reacting T-cells. Here, reverse vaccinology was applied to identify cross-reacting MHC class I T-cell epitopes from two different SwIV H1 lineages in pigs. In silico prediction followed by in vitro and in vivo testing was used to identify SLA-1*0702 T-cell epitopes in heterologous SwIV-infected pigs. Following viral infection, tetramer specific T-cell populations were identified. The majority of the identified T-cell epitopes were conserved between the examined lineages, suggesting that targeting cross-reactive T-cell epitopes could be used to improve vaccines against SwIV in SLA-1*0702-positive pigs.

KW - Cross-reactive epitopes

KW - MHC class I T cell epitopes

KW - Reverse vaccinology

KW - Swine influenza virus

U2 - 10.1099/jgv.0.000748

DO - 10.1099/jgv.0.000748

M3 - Journal article

C2 - 28555545

AN - SCOPUS:85020395272

VL - 98

SP - 895

EP - 899

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 5

M1 - 000748

ER -

ID: 247394601