Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies

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Standard

Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a) : cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. / Petersen, Cathrine Bie; Hillig, Ann-Britt Nygaard; Viuff, Birgitte; Fredholm, Merete; Aasted, Bent; Salomonsen, Jan.

I: Developmental & Comparative Immunology, Bind 31, Nr. 6, 2007, s. 618-631.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Petersen, CB, Hillig, A-BN, Viuff, B, Fredholm, M, Aasted, B & Salomonsen, J 2007, 'Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies', Developmental & Comparative Immunology, bind 31, nr. 6, s. 618-631. https://doi.org/10.1016/j.dci.2006.08.012

APA

Petersen, C. B., Hillig, A-B. N., Viuff, B., Fredholm, M., Aasted, B., & Salomonsen, J. (2007). Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. Developmental & Comparative Immunology, 31(6), 618-631. https://doi.org/10.1016/j.dci.2006.08.012

Vancouver

Petersen CB, Hillig A-BN, Viuff B, Fredholm M, Aasted B, Salomonsen J. Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. Developmental & Comparative Immunology. 2007;31(6):618-631. https://doi.org/10.1016/j.dci.2006.08.012

Author

Petersen, Cathrine Bie ; Hillig, Ann-Britt Nygaard ; Viuff, Birgitte ; Fredholm, Merete ; Aasted, Bent ; Salomonsen, Jan. / Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a) : cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. I: Developmental & Comparative Immunology. 2007 ; Bind 31, Nr. 6. s. 618-631.

Bibtex

@article{64b07970a1c111ddb6ae000ea68e967b,
title = "Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies",
abstract = "Swine workshop cluster 9 (SWC9) antibody identifying a porcien epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids.",
keywords = "Former LIFE faculty, SWC9, NPP1, Swine, Macrophage",
author = "Petersen, {Cathrine Bie} and Hillig, {Ann-Britt Nygaard} and Birgitte Viuff and Merete Fredholm and Bent Aasted and Jan Salomonsen",
year = "2007",
doi = "10.1016/j.dci.2006.08.012",
language = "English",
volume = "31",
pages = "618--631",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Pergamon",
number = "6",

}

RIS

TY - JOUR

T1 - Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a)

T2 - cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies

AU - Petersen, Cathrine Bie

AU - Hillig, Ann-Britt Nygaard

AU - Viuff, Birgitte

AU - Fredholm, Merete

AU - Aasted, Bent

AU - Salomonsen, Jan

PY - 2007

Y1 - 2007

N2 - Swine workshop cluster 9 (SWC9) antibody identifying a porcien epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids.

AB - Swine workshop cluster 9 (SWC9) antibody identifying a porcien epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids.

KW - Former LIFE faculty

KW - SWC9

KW - NPP1

KW - Swine

KW - Macrophage

U2 - 10.1016/j.dci.2006.08.012

DO - 10.1016/j.dci.2006.08.012

M3 - Journal article

C2 - 17064772

VL - 31

SP - 618

EP - 631

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

IS - 6

ER -

ID: 8043334