The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA

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Standard

The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA. / Gottschalk, Sanne; Thomsen, Line Elnif.

Antimicrobial Peptides. Bind 1548 Humana Press, 2017. s. 297-305 (Methods in Molecular Biology, Bind 1548).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Gottschalk, S & Thomsen, LE 2017, The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA. i Antimicrobial Peptides. bind 1548, Humana Press, Methods in Molecular Biology, bind 1548, s. 297-305. https://doi.org/10.1007/978-1-4939-6737-7_21

APA

Gottschalk, S., & Thomsen, L. E. (2017). The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA. I Antimicrobial Peptides (Bind 1548, s. 297-305). Humana Press. Methods in Molecular Biology Bind 1548 https://doi.org/10.1007/978-1-4939-6737-7_21

Vancouver

Gottschalk S, Thomsen LE. The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA. I Antimicrobial Peptides. Bind 1548. Humana Press. 2017. s. 297-305. (Methods in Molecular Biology, Bind 1548). https://doi.org/10.1007/978-1-4939-6737-7_21

Author

Gottschalk, Sanne ; Thomsen, Line Elnif. / The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA. Antimicrobial Peptides. Bind 1548 Humana Press, 2017. s. 297-305 (Methods in Molecular Biology, Bind 1548).

Bibtex

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title = "The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA",
abstract = "The analysis of how antimicrobial peptides (AMPs) interact with bacterial membranes and intracellular targets is important for our understanding of how these molecules affect bacteria. Increased knowledge may aid the design of AMPs that work on their target bacterium without inducing bacterial resistance. Here, we describe different methods to investigate the mode of action of peptides against the Gram-positive bacterium Staphylococcus aureus. ATP leakage analysis can be used to evaluate the ability of AMPs to perturb bacteria. DNA-binding and SOS response induction can be analyzed to investigate intracellular targets.",
keywords = "Antimicrobial peptides, ATP leakage, DNA binding, Membrane perturbation, Mode of action, SOS response",
author = "Sanne Gottschalk and Thomsen, {Line Elnif}",
year = "2017",
doi = "10.1007/978-1-4939-6737-7_21",
language = "English",
isbn = "978-1-4939-6735-3",
volume = "1548",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "297--305",
booktitle = "Antimicrobial Peptides",
address = "United States",

}

RIS

TY - CHAP

T1 - The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA

AU - Gottschalk, Sanne

AU - Thomsen, Line Elnif

PY - 2017

Y1 - 2017

N2 - The analysis of how antimicrobial peptides (AMPs) interact with bacterial membranes and intracellular targets is important for our understanding of how these molecules affect bacteria. Increased knowledge may aid the design of AMPs that work on their target bacterium without inducing bacterial resistance. Here, we describe different methods to investigate the mode of action of peptides against the Gram-positive bacterium Staphylococcus aureus. ATP leakage analysis can be used to evaluate the ability of AMPs to perturb bacteria. DNA-binding and SOS response induction can be analyzed to investigate intracellular targets.

AB - The analysis of how antimicrobial peptides (AMPs) interact with bacterial membranes and intracellular targets is important for our understanding of how these molecules affect bacteria. Increased knowledge may aid the design of AMPs that work on their target bacterium without inducing bacterial resistance. Here, we describe different methods to investigate the mode of action of peptides against the Gram-positive bacterium Staphylococcus aureus. ATP leakage analysis can be used to evaluate the ability of AMPs to perturb bacteria. DNA-binding and SOS response induction can be analyzed to investigate intracellular targets.

KW - Antimicrobial peptides

KW - ATP leakage

KW - DNA binding

KW - Membrane perturbation

KW - Mode of action

KW - SOS response

U2 - 10.1007/978-1-4939-6737-7_21

DO - 10.1007/978-1-4939-6737-7_21

M3 - Book chapter

C2 - 28013513

AN - SCOPUS:85007495047

SN - 978-1-4939-6735-3

VL - 1548

T3 - Methods in Molecular Biology

SP - 297

EP - 305

BT - Antimicrobial Peptides

PB - Humana Press

ER -

ID: 188416444