Antibacterial effects of the lectin from pomegranate sarcotesta (PgTeL) against Listeria monocytogenes
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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Antibacterial effects of the lectin from pomegranate sarcotesta (PgTeL) against Listeria monocytogenes. / Silva, P. M.; Silva, J. N.O.; Silva, B. R.; Ferreira, G. R.S.; Gaião, W. D.C.; Recio, M. V.; Gonçalves, G. G.A.; Rodrigues, C. G.; Medeiros, P. L.; Brayner, F. A.; Alves, L. C.; Larsen, M. H.; Ingmer, H.; Napoleão, T. H.; Paiva, P. M.G.
I: Journal of Applied Microbiology, Bind 131, Nr. 2, 2021, s. 671-681.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Antibacterial effects of the lectin from pomegranate sarcotesta (PgTeL) against Listeria monocytogenes
AU - Silva, P. M.
AU - Silva, J. N.O.
AU - Silva, B. R.
AU - Ferreira, G. R.S.
AU - Gaião, W. D.C.
AU - Recio, M. V.
AU - Gonçalves, G. G.A.
AU - Rodrigues, C. G.
AU - Medeiros, P. L.
AU - Brayner, F. A.
AU - Alves, L. C.
AU - Larsen, M. H.
AU - Ingmer, H.
AU - Napoleão, T. H.
AU - Paiva, P. M.G.
PY - 2021
Y1 - 2021
N2 - Aims: To investigate the effects of the lectin from Punica granatum sarcotesta (PgTeL) on growth, viability, cell structure, biofilm formation and chitinase activity of Listeria monocytogenes. In addition, the effect of PgTeL on the adhesion and invasion of human cells (HeLa) was determined. Methods and Results: PgTeL showed bacteriostatic and bactericidal effects on the strains L. monocytogenes N53-1 and EGD-e, causing morphometric alterations, cell aggregation, strong deformation and cell disruption. PgTeL inhibited biofilm formation by EGD-e and N53-1 and also interfered with the adhesion and invasion processes of EGD-e and N53-1 in HeLa cells. Finally, the chitinase activity of L. monocytogenes EGD-e was reduced in the presence of PgTeL, which can be involved in the inhibition of adhesion process. Conclusion: PgTeL is an antibacterial agent against L. monocytogenes, inhibiting growth and promoting cell death, as well as impairing biofilm formation and bacterial adhesion and invasion into human cells. Significance and Impact of the Study: The results stimulate future investigations on the potential of PgTeL for protection of contamination in food products.
AB - Aims: To investigate the effects of the lectin from Punica granatum sarcotesta (PgTeL) on growth, viability, cell structure, biofilm formation and chitinase activity of Listeria monocytogenes. In addition, the effect of PgTeL on the adhesion and invasion of human cells (HeLa) was determined. Methods and Results: PgTeL showed bacteriostatic and bactericidal effects on the strains L. monocytogenes N53-1 and EGD-e, causing morphometric alterations, cell aggregation, strong deformation and cell disruption. PgTeL inhibited biofilm formation by EGD-e and N53-1 and also interfered with the adhesion and invasion processes of EGD-e and N53-1 in HeLa cells. Finally, the chitinase activity of L. monocytogenes EGD-e was reduced in the presence of PgTeL, which can be involved in the inhibition of adhesion process. Conclusion: PgTeL is an antibacterial agent against L. monocytogenes, inhibiting growth and promoting cell death, as well as impairing biofilm formation and bacterial adhesion and invasion into human cells. Significance and Impact of the Study: The results stimulate future investigations on the potential of PgTeL for protection of contamination in food products.
KW - biofilm
KW - food-borne infection
KW - lectin
KW - Listeria monocytogenes
KW - pomegranate juice
U2 - 10.1111/jam.14978
DO - 10.1111/jam.14978
M3 - Journal article
C2 - 33342053
AN - SCOPUS:85099019444
VL - 131
SP - 671
EP - 681
JO - Proceedings of the Society for Applied Bacteriology
JF - Proceedings of the Society for Applied Bacteriology
SN - 0370-1778
IS - 2
ER -
ID: 259675302