Characterisation of Salmonella enteritidis ST11 and ST1925 Associated with Human Intestinal and Extra-Intestinal Infections in Singapore

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Standard

Characterisation of Salmonella enteritidis ST11 and ST1925 Associated with Human Intestinal and Extra-Intestinal Infections in Singapore. / Aung, Kyaw Thu; Khor, Wei Ching; Ong, Kar Hui; Tan, Wei Ling; Wong, Zhi Ning; Oh, Jia Quan; Wong, Wai Kwan; Tan, Brian Zi Yan; Maiwald, Matthias; Tee, Nancy Wen Sim; Barkham, Timothy; Koh, Tse Hsien; Dalsgaard, Anders; Chen, Swaine L.; Schlundt, Joergen; Ng, Lee Ching.

I: International Journal of Environmental Research and Public Health, Bind 19, Nr. 9, 5671, 2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Aung, KT, Khor, WC, Ong, KH, Tan, WL, Wong, ZN, Oh, JQ, Wong, WK, Tan, BZY, Maiwald, M, Tee, NWS, Barkham, T, Koh, TH, Dalsgaard, A, Chen, SL, Schlundt, J & Ng, LC 2022, 'Characterisation of Salmonella enteritidis ST11 and ST1925 Associated with Human Intestinal and Extra-Intestinal Infections in Singapore', International Journal of Environmental Research and Public Health, bind 19, nr. 9, 5671. https://doi.org/10.3390/ijerph19095671

APA

Aung, K. T., Khor, W. C., Ong, K. H., Tan, W. L., Wong, Z. N., Oh, J. Q., Wong, W. K., Tan, B. Z. Y., Maiwald, M., Tee, N. W. S., Barkham, T., Koh, T. H., Dalsgaard, A., Chen, S. L., Schlundt, J., & Ng, L. C. (2022). Characterisation of Salmonella enteritidis ST11 and ST1925 Associated with Human Intestinal and Extra-Intestinal Infections in Singapore. International Journal of Environmental Research and Public Health, 19(9), [5671]. https://doi.org/10.3390/ijerph19095671

Vancouver

Aung KT, Khor WC, Ong KH, Tan WL, Wong ZN, Oh JQ o.a. Characterisation of Salmonella enteritidis ST11 and ST1925 Associated with Human Intestinal and Extra-Intestinal Infections in Singapore. International Journal of Environmental Research and Public Health. 2022;19(9). 5671. https://doi.org/10.3390/ijerph19095671

Author

Aung, Kyaw Thu ; Khor, Wei Ching ; Ong, Kar Hui ; Tan, Wei Ling ; Wong, Zhi Ning ; Oh, Jia Quan ; Wong, Wai Kwan ; Tan, Brian Zi Yan ; Maiwald, Matthias ; Tee, Nancy Wen Sim ; Barkham, Timothy ; Koh, Tse Hsien ; Dalsgaard, Anders ; Chen, Swaine L. ; Schlundt, Joergen ; Ng, Lee Ching. / Characterisation of Salmonella enteritidis ST11 and ST1925 Associated with Human Intestinal and Extra-Intestinal Infections in Singapore. I: International Journal of Environmental Research and Public Health. 2022 ; Bind 19, Nr. 9.

Bibtex

@article{619cddeedf134f22802b9931d8f40f2c,
title = "Characterisation of Salmonella enteritidis ST11 and ST1925 Associated with Human Intestinal and Extra-Intestinal Infections in Singapore",
abstract = "Salmonella enteritidis is a major foodborne pathogen worldwide. In this study, a total of 276 S. enteritidis isolates, collected between 2016 and 2017 from human, food and farm/slaughterhouse samples, were studied to enhance the understanding of the epidemiology of human salmonellosis in Singapore. Results showed all 276 isolates belonged either to ST1925 (70.3%) or ST11 (29.7%), with ST11 being significantly more frequent in extra-intestinal isolates and chicken isolates. Food isolates, most of which were from poultry, showed the highest prevalence of resistance (33–37%) against beta-lactams or beta-lactams/beta-lactamase inhibitor combination (ampicillin, piperacillin and ampicillin/sulbactam). The analysis showed the detection of genes associated with resistance to aminoglycoside genes (99.6%), tetracycline (55.1%), and beta-lactams (14.9%) of all isolates. Nine types of plasmids were found in 266 isolates; the most common incompatibility group profiles were IncFIB(S)-IncFII(S)-IncX1 (72.2%) and IncFIB(S)-IncFII(S) (15.8%). Most plasmid harbouring isolates from chicken (63.6%, 14/22) and from human (73.8%, 175/237) shared the same plasmid profile (IncFIB(S)-IncFII(S)-IncX1). SNP analysis showed clustering of several isolates from poultry food products and human isolates, suggesting phylogenetic relatedness among these isolates. Lastly, this study provides important epidemiological insights on the application of phenotypic and next-generation sequencing (NGS) tools for improved food safety and public health surveillance and outbreak investigation of S. enteritidis.",
keywords = "antimicrobial resistance, epidemiology, farm environment, food, human, integrated surveillance, Salmonella enteritidis, salmonellosis, sequence type",
author = "Aung, {Kyaw Thu} and Khor, {Wei Ching} and Ong, {Kar Hui} and Tan, {Wei Ling} and Wong, {Zhi Ning} and Oh, {Jia Quan} and Wong, {Wai Kwan} and Tan, {Brian Zi Yan} and Matthias Maiwald and Tee, {Nancy Wen Sim} and Timothy Barkham and Koh, {Tse Hsien} and Anders Dalsgaard and Chen, {Swaine L.} and Joergen Schlundt and Ng, {Lee Ching}",
note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
doi = "10.3390/ijerph19095671",
language = "English",
volume = "19",
journal = "International Journal of Environmental Research and Public Health",
issn = "1661-7827",
publisher = "MDPI AG",
number = "9",

}

RIS

TY - JOUR

T1 - Characterisation of Salmonella enteritidis ST11 and ST1925 Associated with Human Intestinal and Extra-Intestinal Infections in Singapore

AU - Aung, Kyaw Thu

AU - Khor, Wei Ching

AU - Ong, Kar Hui

AU - Tan, Wei Ling

AU - Wong, Zhi Ning

AU - Oh, Jia Quan

AU - Wong, Wai Kwan

AU - Tan, Brian Zi Yan

AU - Maiwald, Matthias

AU - Tee, Nancy Wen Sim

AU - Barkham, Timothy

AU - Koh, Tse Hsien

AU - Dalsgaard, Anders

AU - Chen, Swaine L.

AU - Schlundt, Joergen

AU - Ng, Lee Ching

N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022

Y1 - 2022

N2 - Salmonella enteritidis is a major foodborne pathogen worldwide. In this study, a total of 276 S. enteritidis isolates, collected between 2016 and 2017 from human, food and farm/slaughterhouse samples, were studied to enhance the understanding of the epidemiology of human salmonellosis in Singapore. Results showed all 276 isolates belonged either to ST1925 (70.3%) or ST11 (29.7%), with ST11 being significantly more frequent in extra-intestinal isolates and chicken isolates. Food isolates, most of which were from poultry, showed the highest prevalence of resistance (33–37%) against beta-lactams or beta-lactams/beta-lactamase inhibitor combination (ampicillin, piperacillin and ampicillin/sulbactam). The analysis showed the detection of genes associated with resistance to aminoglycoside genes (99.6%), tetracycline (55.1%), and beta-lactams (14.9%) of all isolates. Nine types of plasmids were found in 266 isolates; the most common incompatibility group profiles were IncFIB(S)-IncFII(S)-IncX1 (72.2%) and IncFIB(S)-IncFII(S) (15.8%). Most plasmid harbouring isolates from chicken (63.6%, 14/22) and from human (73.8%, 175/237) shared the same plasmid profile (IncFIB(S)-IncFII(S)-IncX1). SNP analysis showed clustering of several isolates from poultry food products and human isolates, suggesting phylogenetic relatedness among these isolates. Lastly, this study provides important epidemiological insights on the application of phenotypic and next-generation sequencing (NGS) tools for improved food safety and public health surveillance and outbreak investigation of S. enteritidis.

AB - Salmonella enteritidis is a major foodborne pathogen worldwide. In this study, a total of 276 S. enteritidis isolates, collected between 2016 and 2017 from human, food and farm/slaughterhouse samples, were studied to enhance the understanding of the epidemiology of human salmonellosis in Singapore. Results showed all 276 isolates belonged either to ST1925 (70.3%) or ST11 (29.7%), with ST11 being significantly more frequent in extra-intestinal isolates and chicken isolates. Food isolates, most of which were from poultry, showed the highest prevalence of resistance (33–37%) against beta-lactams or beta-lactams/beta-lactamase inhibitor combination (ampicillin, piperacillin and ampicillin/sulbactam). The analysis showed the detection of genes associated with resistance to aminoglycoside genes (99.6%), tetracycline (55.1%), and beta-lactams (14.9%) of all isolates. Nine types of plasmids were found in 266 isolates; the most common incompatibility group profiles were IncFIB(S)-IncFII(S)-IncX1 (72.2%) and IncFIB(S)-IncFII(S) (15.8%). Most plasmid harbouring isolates from chicken (63.6%, 14/22) and from human (73.8%, 175/237) shared the same plasmid profile (IncFIB(S)-IncFII(S)-IncX1). SNP analysis showed clustering of several isolates from poultry food products and human isolates, suggesting phylogenetic relatedness among these isolates. Lastly, this study provides important epidemiological insights on the application of phenotypic and next-generation sequencing (NGS) tools for improved food safety and public health surveillance and outbreak investigation of S. enteritidis.

KW - antimicrobial resistance

KW - epidemiology

KW - farm environment

KW - food

KW - human

KW - integrated surveillance

KW - Salmonella enteritidis

KW - salmonellosis

KW - sequence type

U2 - 10.3390/ijerph19095671

DO - 10.3390/ijerph19095671

M3 - Journal article

C2 - 35565065

AN - SCOPUS:85129442077

VL - 19

JO - International Journal of Environmental Research and Public Health

JF - International Journal of Environmental Research and Public Health

SN - 1661-7827

IS - 9

M1 - 5671

ER -

ID: 308129088