TY - JOUR

T1 - Genomic and Phenotypic Insights for Toxigenic Clinical Vibrio cholerae O141

AU - Hounmanou, Yaovi M.G.

AU - Sit, Brandon

AU - Fakoya, Bolutife

AU - Waldor, Matthew K.

AU - Dalsgaard, Anders

N1 - Publisher Copyright: © 2022 Centers for Disease Control and Prevention (CDC). All rights reserved.

PY - 2022

Y1 - 2022

N2 - Vibrio cholerae remains a major public health threat worldwide, causing millions of cholera cases each year. Although much is known about the evolution and pathogenicity of the O1/O139 serogroups of V. cholerae, information is lacking on the molecular epidemiology of non-O1/O139 strains isolated from patients who have diarrheal illnesses. We performed whole-genome sequence analysis and in vivo infections to investigate characteristics of V. cholerae O141 isolated from sporadic diarrheal cases in 4 countries. The strains formed a distinct phylogenetic clade distinguishable from other serogroups and a unique multilocus sequence type 42, but interstrain variation suggests that O141 isolates are not clonal. These isolates encode virulence factors including cholera toxin and the toxin-coregulated pilus, as well as a type 3 secretion system. They had widely variable capacities for intestinal colonization in the infant mouse model. We propose that O141 isolates comprise a distinct clade of V. cholerae non-O1/O139, and their continued surveillance is warranted.

AB - Vibrio cholerae remains a major public health threat worldwide, causing millions of cholera cases each year. Although much is known about the evolution and pathogenicity of the O1/O139 serogroups of V. cholerae, information is lacking on the molecular epidemiology of non-O1/O139 strains isolated from patients who have diarrheal illnesses. We performed whole-genome sequence analysis and in vivo infections to investigate characteristics of V. cholerae O141 isolated from sporadic diarrheal cases in 4 countries. The strains formed a distinct phylogenetic clade distinguishable from other serogroups and a unique multilocus sequence type 42, but interstrain variation suggests that O141 isolates are not clonal. These isolates encode virulence factors including cholera toxin and the toxin-coregulated pilus, as well as a type 3 secretion system. They had widely variable capacities for intestinal colonization in the infant mouse model. We propose that O141 isolates comprise a distinct clade of V. cholerae non-O1/O139, and their continued surveillance is warranted.

U2 - 10.3201/eid2803.210715

DO - 10.3201/eid2803.210715

M3 - Journal article

C2 - 35202520

AN - SCOPUS:85125170322

VL - 28

SP - 617

EP - 624

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

SN - 1080-6040

IS - 3

ER -