TY - JOUR

T1 - Pharmacokinetics and muscle residue depletion of amoxicillin in cage cultured hybrid red tilapia (Oreochromis mossambicus × Oreochromis niloticus)

AU - Phu, Tran Minh

AU - Em, Nguyen Tam

AU - Thinh, Nguyen Quoc

AU - Phuong, Nguyen Thanh

AU - Dalgaard, Anders

AU - Scippo, Marie Louise

AU - Devreese, Mathias

AU - Croubels, Siska

PY - 2019

Y1 - 2019

N2 - Amoxicillin (AMX) is commonly used to treat Streptococcus spp. infection in hybrid red tilapia (Oreochromis mossambicus × Oreochromis niloticus), despite the lack of plasma pharmacokinetic data, and suitable edible tissue residue withdrawal time estimations. The aim of this study was therefore to investigate plasma pharmacokinetics and muscle tissue residues depletion of AMX in hybrid red tilapia raised in cages. Cage raised hybrid red tilapia (223 ± 11.5 g) from Southern Vietnam were treated with medicated feed containing AMX at a dose of 5000 mg/kg feed (equal to 50 mg/kg body weight fish) for five consecutive days. During administration of the medicated feed, fish muscle (including skin) samples were taken at days 1 and 5 (each time at 2 h, 12 h and 24 h after feeding) and at 3, 7 and 14 days after the cessation of medicated feed administration. Plasma was collected at 30 min, and at 1, 2, 6, 12 and 24 h after the first medication and at 2, 12 and 24 h after administration of medicated feed on day 2, 3, 4 and 5. AMX levels were determined by liquid chromatography coupled to tandem mass spectrometry. Results showed that AMX depleted rapidly in fish muscle and skin and was below the limit of quantification (5 μg/kg) within 24 h after five consecutive days of medication. Noncompartmental pharmacokinetic analysis of AMX revealed that maximum plasma concentration (C max ) on day 1 was 2.75 ± 0.98 μg/mL (T max , 1 h), whereas it ranged from 0.81 to 0.97 μg/mL (day 2 to 4) and decreased to 0.27 ± 0.07 μg/mL (T max 2–12 h) on day five of medication. Also, the highest area under the plasma concentration-time curve from time 0 to 24 h (AUC 0–24h ; 28.06 ± 3.32 h·μg/mL) was found on the first day of treatment and decreased on the following days. The elimination rate constant (k el ) was 0.17 h −1 and elimination half-life (T 1/2el ) was 4.1 h. Our results show that AMX is depleted from edible muscle tissue in a short time period, which suggests a low, if any, food safety hazard associated with common treatment regimes. However, further research is needed to define a well-suited therapeutic dose.

AB - Amoxicillin (AMX) is commonly used to treat Streptococcus spp. infection in hybrid red tilapia (Oreochromis mossambicus × Oreochromis niloticus), despite the lack of plasma pharmacokinetic data, and suitable edible tissue residue withdrawal time estimations. The aim of this study was therefore to investigate plasma pharmacokinetics and muscle tissue residues depletion of AMX in hybrid red tilapia raised in cages. Cage raised hybrid red tilapia (223 ± 11.5 g) from Southern Vietnam were treated with medicated feed containing AMX at a dose of 5000 mg/kg feed (equal to 50 mg/kg body weight fish) for five consecutive days. During administration of the medicated feed, fish muscle (including skin) samples were taken at days 1 and 5 (each time at 2 h, 12 h and 24 h after feeding) and at 3, 7 and 14 days after the cessation of medicated feed administration. Plasma was collected at 30 min, and at 1, 2, 6, 12 and 24 h after the first medication and at 2, 12 and 24 h after administration of medicated feed on day 2, 3, 4 and 5. AMX levels were determined by liquid chromatography coupled to tandem mass spectrometry. Results showed that AMX depleted rapidly in fish muscle and skin and was below the limit of quantification (5 μg/kg) within 24 h after five consecutive days of medication. Noncompartmental pharmacokinetic analysis of AMX revealed that maximum plasma concentration (C max ) on day 1 was 2.75 ± 0.98 μg/mL (T max , 1 h), whereas it ranged from 0.81 to 0.97 μg/mL (day 2 to 4) and decreased to 0.27 ± 0.07 μg/mL (T max 2–12 h) on day five of medication. Also, the highest area under the plasma concentration-time curve from time 0 to 24 h (AUC 0–24h ; 28.06 ± 3.32 h·μg/mL) was found on the first day of treatment and decreased on the following days. The elimination rate constant (k el ) was 0.17 h −1 and elimination half-life (T 1/2el ) was 4.1 h. Our results show that AMX is depleted from edible muscle tissue in a short time period, which suggests a low, if any, food safety hazard associated with common treatment regimes. However, further research is needed to define a well-suited therapeutic dose.

KW - Amoxicillin

KW - Hybrid red tilapia

KW - Pharmacokinetics

KW - Tissue residues

KW - Withdrawal time

U2 - 10.1016/j.aquaculture.2019.02.042

DO - 10.1016/j.aquaculture.2019.02.042

M3 - Journal article

AN - SCOPUS:85062147051

VL - 505

SP - 206

EP - 211

JO - Aquaculture

JF - Aquaculture

SN - 0044-8486

ER -