Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride

Institut for Veterinær- og Husdyrvidenskab

Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride. / Woudstra, Cedric; Brøndsted, Lone.

I: PHAGE: Therapy, Applications, and Research, Bind 4, Nr. 3, 2023, s. 136-140.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Woudstra, C & Brøndsted, L 2023, 'Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride', PHAGE: Therapy, Applications, and Research, bind 4, nr. 3, s. 136-140. https://doi.org/10.1089/phage.2023.0014

APA

Woudstra, C., & Brøndsted, L. (2023). Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride. PHAGE: Therapy, Applications, and Research, 4(3), 136-140. https://doi.org/10.1089/phage.2023.0014

Vancouver

Woudstra C, Brøndsted L. Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride. PHAGE: Therapy, Applications, and Research. 2023;4(3):136-140. https://doi.org/10.1089/phage.2023.0014

Author

Woudstra, Cedric ; Brøndsted, Lone. / Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride. I: PHAGE: Therapy, Applications, and Research. 2023 ; Bind 4, Nr. 3. s. 136-140.

Bibtex

@article{524c1ccf6ff04b509b20226c80d66484,

title = "Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride",

abstract = "In the light of the worldwide antimicrobial resistance crisis, new substitutes to antibiotics are urgently needed. Tailocins or phage tail-like bacteriocin particles, produced by bacteria for environmental competition, are a potential antimicrobial alternative to antibiotic treatment. Yet, the availability of characterized Tailocins is limited. We explored the possibility to produce new Tailocins from phage particles, using osmotic shock or chemical treatment by the ammonium quaternary compound benzalkonium chloride on Ackermannviridae phage S117 and using Straboviridae phage T4 as control. We report that phage S117 was resistant to such treatment, while successful production of Tailocins by osmotic shock was achieved for phage T4. Finally, chemical treatment with benzalkonium chloride was inefficient on phage S117 but successfully inactivated phage T4 without production of Tailocins. Further studies are needed to implement such treatments of phages for producing Tailocins with killing activity. ",

keywords = "antimicrobial, Bacteriophage, head-less phage, osmotic shock, phage tail-like particle, quaternary compound, Tailocin",

author = "Cedric Woudstra and Lone Br{\o}ndsted",

year = "2023",

doi = "10.1089/phage.2023.0014",

language = "English",

volume = "4",

pages = "136--140",

journal = "PHAGE: Therapy, Applications, and Research",

issn = "2641-6530",

publisher = "Mary AnnLiebert, Inc. Publishers",

number = "3",

}

RIS

TY - JOUR

T1 - Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride

AU - Woudstra, Cedric

AU - Brøndsted, Lone

PY - 2023

Y1 - 2023

N2 - In the light of the worldwide antimicrobial resistance crisis, new substitutes to antibiotics are urgently needed. Tailocins or phage tail-like bacteriocin particles, produced by bacteria for environmental competition, are a potential antimicrobial alternative to antibiotic treatment. Yet, the availability of characterized Tailocins is limited. We explored the possibility to produce new Tailocins from phage particles, using osmotic shock or chemical treatment by the ammonium quaternary compound benzalkonium chloride on Ackermannviridae phage S117 and using Straboviridae phage T4 as control. We report that phage S117 was resistant to such treatment, while successful production of Tailocins by osmotic shock was achieved for phage T4. Finally, chemical treatment with benzalkonium chloride was inefficient on phage S117 but successfully inactivated phage T4 without production of Tailocins. Further studies are needed to implement such treatments of phages for producing Tailocins with killing activity.

AB - In the light of the worldwide antimicrobial resistance crisis, new substitutes to antibiotics are urgently needed. Tailocins or phage tail-like bacteriocin particles, produced by bacteria for environmental competition, are a potential antimicrobial alternative to antibiotic treatment. Yet, the availability of characterized Tailocins is limited. We explored the possibility to produce new Tailocins from phage particles, using osmotic shock or chemical treatment by the ammonium quaternary compound benzalkonium chloride on Ackermannviridae phage S117 and using Straboviridae phage T4 as control. We report that phage S117 was resistant to such treatment, while successful production of Tailocins by osmotic shock was achieved for phage T4. Finally, chemical treatment with benzalkonium chloride was inefficient on phage S117 but successfully inactivated phage T4 without production of Tailocins. Further studies are needed to implement such treatments of phages for producing Tailocins with killing activity.

KW - antimicrobial

KW - Bacteriophage

KW - head-less phage

KW - osmotic shock

KW - phage tail-like particle

KW - quaternary compound

KW - Tailocin

U2 - 10.1089/phage.2023.0014

DO - 10.1089/phage.2023.0014

M3 - Journal article

C2 - 37841391

AN - SCOPUS:85173731951

VL - 4

SP - 136

EP - 140

JO - PHAGE: Therapy, Applications, and Research

JF - PHAGE: Therapy, Applications, and Research

SN - 2641-6530

IS - 3

ER -

ID: 372581553