Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Standard
Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus. / Bojer, Martin Saxtorph; Baldry, Mara; Ingmer, Hanne.
Antimicrobial Peptides: Methods and Protocols. red. / Paul R. Hansen. Bind 1548 Humana Press, 2017. s. 387-394 (Methods in Molecular Biology, Bind 1548).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - CHAP
T1 - Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus
AU - Bojer, Martin Saxtorph
AU - Baldry, Mara
AU - Ingmer, Hanne
PY - 2017
Y1 - 2017
N2 - Compounds that inhibit virulence gene expression in bacterial pathogens have received increasing interest as possible alternatives to the traditional antibiotic treatment of infections. For the human pathogen Staphylococcus aureus, we have developed two simple assays based on reporter gene fusions to central virulence genes that are easily applicable for screening various sources of natural and synthetic peptides for anti-virulence effects. The plate assay is qualitative but simultaneously assesses the effect of gradient concentrations of the investigated compound, whereas the liquid assay is quantitative and can be employed to address whether a compound is acting on the central quorum sensing regulatory system, agr, that controls a large number of virulence genes in S. aureus.
AB - Compounds that inhibit virulence gene expression in bacterial pathogens have received increasing interest as possible alternatives to the traditional antibiotic treatment of infections. For the human pathogen Staphylococcus aureus, we have developed two simple assays based on reporter gene fusions to central virulence genes that are easily applicable for screening various sources of natural and synthetic peptides for anti-virulence effects. The plate assay is qualitative but simultaneously assesses the effect of gradient concentrations of the investigated compound, whereas the liquid assay is quantitative and can be employed to address whether a compound is acting on the central quorum sensing regulatory system, agr, that controls a large number of virulence genes in S. aureus.
KW - Agr quorum sensing
KW - Anti-virulence
KW - Compound screening
KW - Hla
KW - Peptides
KW - RNAIII
KW - S. aureus
KW - Spa
KW - β-Galactosidase/β-lactamase reporter genes
U2 - 10.1007/978-1-4939-6737-7_28
DO - 10.1007/978-1-4939-6737-7_28
M3 - Book chapter
C2 - 28013520
AN - SCOPUS:85007483207
SN - 978-1-4939-6735-3
VL - 1548
T3 - Methods in Molecular Biology
SP - 387
EP - 394
BT - Antimicrobial Peptides
A2 - Hansen, Paul R.
PB - Humana Press
ER -
ID: 182092202