Rapid in vivo development of resistance to daptomycin in vancomycin-resistant Enterococcus faecium due to genomic alterations

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Standard

Rapid in vivo development of resistance to daptomycin in vancomycin-resistant Enterococcus faecium due to genomic alterations. / Mollerup, Sarah; Elmeskov, Christine; Pinholt, Mette; Sejersen, Tobias S.; Pedersen, Martin S.; Worning, Peder; Frees, Dorte; Westh, Henrik.

I: FEMS Microbiology Letters, Bind 369, Nr. 1, 063, 2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Mollerup, S, Elmeskov, C, Pinholt, M, Sejersen, TS, Pedersen, MS, Worning, P, Frees, D & Westh, H 2022, 'Rapid in vivo development of resistance to daptomycin in vancomycin-resistant Enterococcus faecium due to genomic alterations', FEMS Microbiology Letters, bind 369, nr. 1, 063. https://doi.org/10.1093/femsle/fnac063

APA

Mollerup, S., Elmeskov, C., Pinholt, M., Sejersen, T. S., Pedersen, M. S., Worning, P., Frees, D., & Westh, H. (2022). Rapid in vivo development of resistance to daptomycin in vancomycin-resistant Enterococcus faecium due to genomic alterations. FEMS Microbiology Letters, 369(1), [063]. https://doi.org/10.1093/femsle/fnac063

Vancouver

Mollerup S, Elmeskov C, Pinholt M, Sejersen TS, Pedersen MS, Worning P o.a. Rapid in vivo development of resistance to daptomycin in vancomycin-resistant Enterococcus faecium due to genomic alterations. FEMS Microbiology Letters. 2022;369(1). 063. https://doi.org/10.1093/femsle/fnac063

Author

Mollerup, Sarah ; Elmeskov, Christine ; Pinholt, Mette ; Sejersen, Tobias S. ; Pedersen, Martin S. ; Worning, Peder ; Frees, Dorte ; Westh, Henrik. / Rapid in vivo development of resistance to daptomycin in vancomycin-resistant Enterococcus faecium due to genomic alterations. I: FEMS Microbiology Letters. 2022 ; Bind 369, Nr. 1.

Bibtex

@article{d1f9b6072082433d9e4ede4dd1590c30,
title = "Rapid in vivo development of resistance to daptomycin in vancomycin-resistant Enterococcus faecium due to genomic alterations",
abstract = "Genomic alterations and altered cell wall structure in daptomycin-resistant Enterococcus faecium revealed by comparison of isolates before and after exposure to daptomycin.Daptomycin is a cyclic lipopeptide used in the treatment of vancomycin-resistant Enterococcus faecium (VREfm). However, the development of daptomycin-resistant VREfm challenges the treatment of nosocomial VREfm infections. Resistance mechanisms of daptomycin are not fully understood. Here, we analyzed the genomic changes leading to a daptomycin-susceptible VREfm isolate becoming resistant after 50 days of daptomycin and linezolid combination therapy. A total of seven isogenic VREfm isolates from the same patient (daptomycin-susceptible and daptomycin-resistant) were analyzed using Illumina whole genome sequencing, and two isolates were further characterized with Nanopore sequencing. One nonsynonymous SNP in the rpoC gene previously shown to harbor mutations in daptomycin-resistant VREfm was identified in the daptomycin-resistant isolates. Whole genome comparative analysis identified the loss of a 46.5 kb fragment, duplication of a 29.7 kb fragment, and integration of two plasmids upon acquisition of daptomycin resistance. Transmission electron microscopy showed similar alterations in cell morphology and cell wall structure as have previously been described in daptomycin-resistant E. faecalis.",
keywords = "vancomycin resistance, Enterococcus faecium, VRE, daptomycin resistance, rpoC, mannose pathway, cell envelope, READ ALIGNMENT, SUSCEPTIBILITY, MECHANISMS",
author = "Sarah Mollerup and Christine Elmeskov and Mette Pinholt and Sejersen, {Tobias S.} and Pedersen, {Martin S.} and Peder Worning and Dorte Frees and Henrik Westh",
year = "2022",
doi = "10.1093/femsle/fnac063",
language = "English",
volume = "369",
journal = "F E M S Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Rapid in vivo development of resistance to daptomycin in vancomycin-resistant Enterococcus faecium due to genomic alterations

AU - Mollerup, Sarah

AU - Elmeskov, Christine

AU - Pinholt, Mette

AU - Sejersen, Tobias S.

AU - Pedersen, Martin S.

AU - Worning, Peder

AU - Frees, Dorte

AU - Westh, Henrik

PY - 2022

Y1 - 2022

N2 - Genomic alterations and altered cell wall structure in daptomycin-resistant Enterococcus faecium revealed by comparison of isolates before and after exposure to daptomycin.Daptomycin is a cyclic lipopeptide used in the treatment of vancomycin-resistant Enterococcus faecium (VREfm). However, the development of daptomycin-resistant VREfm challenges the treatment of nosocomial VREfm infections. Resistance mechanisms of daptomycin are not fully understood. Here, we analyzed the genomic changes leading to a daptomycin-susceptible VREfm isolate becoming resistant after 50 days of daptomycin and linezolid combination therapy. A total of seven isogenic VREfm isolates from the same patient (daptomycin-susceptible and daptomycin-resistant) were analyzed using Illumina whole genome sequencing, and two isolates were further characterized with Nanopore sequencing. One nonsynonymous SNP in the rpoC gene previously shown to harbor mutations in daptomycin-resistant VREfm was identified in the daptomycin-resistant isolates. Whole genome comparative analysis identified the loss of a 46.5 kb fragment, duplication of a 29.7 kb fragment, and integration of two plasmids upon acquisition of daptomycin resistance. Transmission electron microscopy showed similar alterations in cell morphology and cell wall structure as have previously been described in daptomycin-resistant E. faecalis.

AB - Genomic alterations and altered cell wall structure in daptomycin-resistant Enterococcus faecium revealed by comparison of isolates before and after exposure to daptomycin.Daptomycin is a cyclic lipopeptide used in the treatment of vancomycin-resistant Enterococcus faecium (VREfm). However, the development of daptomycin-resistant VREfm challenges the treatment of nosocomial VREfm infections. Resistance mechanisms of daptomycin are not fully understood. Here, we analyzed the genomic changes leading to a daptomycin-susceptible VREfm isolate becoming resistant after 50 days of daptomycin and linezolid combination therapy. A total of seven isogenic VREfm isolates from the same patient (daptomycin-susceptible and daptomycin-resistant) were analyzed using Illumina whole genome sequencing, and two isolates were further characterized with Nanopore sequencing. One nonsynonymous SNP in the rpoC gene previously shown to harbor mutations in daptomycin-resistant VREfm was identified in the daptomycin-resistant isolates. Whole genome comparative analysis identified the loss of a 46.5 kb fragment, duplication of a 29.7 kb fragment, and integration of two plasmids upon acquisition of daptomycin resistance. Transmission electron microscopy showed similar alterations in cell morphology and cell wall structure as have previously been described in daptomycin-resistant E. faecalis.

KW - vancomycin resistance

KW - Enterococcus faecium

KW - VRE

KW - daptomycin resistance

KW - rpoC

KW - mannose pathway

KW - cell envelope

KW - READ ALIGNMENT

KW - SUSCEPTIBILITY

KW - MECHANISMS

U2 - 10.1093/femsle/fnac063

DO - 10.1093/femsle/fnac063

M3 - Journal article

C2 - 35922088

VL - 369

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

SN - 0378-1097

IS - 1

M1 - 063

ER -

ID: 319407784