Research note: Occurrence of mcr-encoded colistin resistance in Escherichia coli from pigs and pig farm workers in Vietnam
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Research note : Occurrence of mcr-encoded colistin resistance in Escherichia coli from pigs and pig farm workers in Vietnam . / Dang, Son Thi Thanh; Truong, Duong Thi Quy; Olsen, John Elmerdahl; Tran, Nhat Thi; Truong, Giang Thi Huong; Vu, Hue Thi Kim; Dalsgaard, Anders.
I: FEMS Microbes, Bind 1, Nr. 1, xtaa003, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Research note
T2 - Occurrence of mcr-encoded colistin resistance in Escherichia coli from pigs and pig farm workers in Vietnam
AU - Dang, Son Thi Thanh
AU - Truong, Duong Thi Quy
AU - Olsen, John Elmerdahl
AU - Tran, Nhat Thi
AU - Truong, Giang Thi Huong
AU - Vu, Hue Thi Kim
AU - Dalsgaard, Anders
PY - 2020
Y1 - 2020
N2 - WHO considers colistin as a highest priority critically important drug for human health, and occurrence of colistin-resistant bacteria in livestock is of health concern. The current study determined occurrence of colistin-resistant Escherichia coli in pigs and workers at pig farms in Vietnam, and investigated the genetic background for resistance. Colistin-resistant E. coli were detected from pigs in 53/116 (45.7%) farms, and from workers taking care of the pigs in 21/94 (22.3%) farms. Colistin-resistant isolates showed MIC to colistin between 4–16 mg/L, they were multidrug resistant (99%) and resistance was caused by the presence of mcr-1 genes in 97/102 (95.1%) E. coli from pigs and in 31/34 (91.1%) isolates from humans. mcr-1 is considered a plasmid-encoded gene, but this was not confirmed in the current investigation. In total, one pig isolate carried both mcr-1 and mcr-3 genes, whereas mcr-2, mcr-4 and mcr-5 genes were not detected. Shared resistance profiles between pig and human isolates on the same farm was only observed in four farms. The study showed that commensal E. coli from pigs in Vietnam constitute a reservoir for colistin-resitant E. coli, however, further studies are needed to confirm that mcr genes are associated with plasmids and their importance for human health.
AB - WHO considers colistin as a highest priority critically important drug for human health, and occurrence of colistin-resistant bacteria in livestock is of health concern. The current study determined occurrence of colistin-resistant Escherichia coli in pigs and workers at pig farms in Vietnam, and investigated the genetic background for resistance. Colistin-resistant E. coli were detected from pigs in 53/116 (45.7%) farms, and from workers taking care of the pigs in 21/94 (22.3%) farms. Colistin-resistant isolates showed MIC to colistin between 4–16 mg/L, they were multidrug resistant (99%) and resistance was caused by the presence of mcr-1 genes in 97/102 (95.1%) E. coli from pigs and in 31/34 (91.1%) isolates from humans. mcr-1 is considered a plasmid-encoded gene, but this was not confirmed in the current investigation. In total, one pig isolate carried both mcr-1 and mcr-3 genes, whereas mcr-2, mcr-4 and mcr-5 genes were not detected. Shared resistance profiles between pig and human isolates on the same farm was only observed in four farms. The study showed that commensal E. coli from pigs in Vietnam constitute a reservoir for colistin-resitant E. coli, however, further studies are needed to confirm that mcr genes are associated with plasmids and their importance for human health.
U2 - 10.1093/femsmc/xtaa003
DO - 10.1093/femsmc/xtaa003
M3 - Journal article
VL - 1
JO - FEMS Microbes
JF - FEMS Microbes
SN - 2633-6685
IS - 1
M1 - xtaa003
ER -
ID: 259671780