TY - JOUR

T1 - Proteome of human T lymphocytes with treatment of cyclosporine and polysaccharopeptide

T2 - Analysis of significant proteins that manipulate T cells proliferation and immunosuppression

AU - Lee, Cheuk Lun

AU - Jiang, Ping Ping

AU - Sit, Wai Hung

AU - Wan, Jennifer Man Fan

N1 - Funding Information: This work was supported in part by RGC Grants# HKU 7511/03M obtained from The University of Hong Kong and partially supported by the Hong Kong Association for Health Care, Hong Kong, SAR. P.R. China.

PY - 2007/10

Y1 - 2007/10

N2 - The aberrant activation of T lymphocyte proliferation is one of the key events in organ transplant recipients and autoimmune disorders. The present study adopted a gel-based proteomics approach to define the proteins representative of the T cell proliferation and to discover the molecules that play critical roles during the suppression of T cell proliferation. Human T lymphocytes were isolated from healthy donors and primed with phytohemagglutinin (PHA) to undergo proliferation. Two medical fungal products with specific T cell activation inhibitory properties, cyclosporine A (CsA) and polysaccharopeptide (PSP), were used to study the proteins that manipulate T cell proliferation. After demonstrating their similar effects on cell proliferation, cell survival and interleuklin-2 (IL-2) secretion, significant quantitative protein alterations were detected between the CsA- and PSP-treated T cell proteome. These altered proteins were identified by MALDI-TOF and classified into 3 categories: (i) proteins affected by both CsA and PSP, (ii) proteins affected by CsA alone, and (iii) proteins affected by PSP alone. Most of these altered proteins have functional significance in protein degradation, the antioxidant pathway, energy metabolism and immune cell regulation.

AB - The aberrant activation of T lymphocyte proliferation is one of the key events in organ transplant recipients and autoimmune disorders. The present study adopted a gel-based proteomics approach to define the proteins representative of the T cell proliferation and to discover the molecules that play critical roles during the suppression of T cell proliferation. Human T lymphocytes were isolated from healthy donors and primed with phytohemagglutinin (PHA) to undergo proliferation. Two medical fungal products with specific T cell activation inhibitory properties, cyclosporine A (CsA) and polysaccharopeptide (PSP), were used to study the proteins that manipulate T cell proliferation. After demonstrating their similar effects on cell proliferation, cell survival and interleuklin-2 (IL-2) secretion, significant quantitative protein alterations were detected between the CsA- and PSP-treated T cell proteome. These altered proteins were identified by MALDI-TOF and classified into 3 categories: (i) proteins affected by both CsA and PSP, (ii) proteins affected by CsA alone, and (iii) proteins affected by PSP alone. Most of these altered proteins have functional significance in protein degradation, the antioxidant pathway, energy metabolism and immune cell regulation.

KW - Coriolus versicolor

KW - Cyclosporine A

KW - Human T lymphocytes

KW - Polysaccharopeptide

KW - Proteomics

KW - Two dimensional gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=34547153230&partnerID=8YFLogxK

U2 - 10.1016/j.intimp.2007.05.013

DO - 10.1016/j.intimp.2007.05.013

M3 - Journal article

C2 - 17673146

AN - SCOPUS:34547153230

VL - 7

SP - 1311

EP - 1324

JO - International Immunopharmacology

JF - International Immunopharmacology

SN - 1567-5769

IS - 10

ER -