CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis
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CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis. / Kohm, Katharina; Lutz, Veronika Theresa; Friedrich, Ines; Hertel, Robert.
Metagenomics: Methods and Protocols. red. / Wolfgang R. Streit; Rolf Daniel. Humana Press, 2023. s. 205-212 (Methods in Molecular Biology, Bind 2555).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis
AU - Kohm, Katharina
AU - Lutz, Veronika Theresa
AU - Friedrich, Ines
AU - Hertel, Robert
N1 - Publisher Copyright: © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - Phages are viruses of bacteria and have been known for over a century. They do not have a metabolism or protein synthesis machinery and rely on host cells for replication. The model organism Bacillus subtilis has served as a host strain for decades and enabled the isolation of many unique viral strains. However, many viral species representatives remained orphans as no, or only a few, related phages were ever re-isolated. The presented protocol describes how a CRISPR-Cas9 system with an artificial CRISPR-array can be set up and used to discriminate abundant and well-known B. subtilis phage from a host-based metagenome enrichment. The obtained viral suspension can be used for metagenome sequencing and isolating new viral strains.
AB - Phages are viruses of bacteria and have been known for over a century. They do not have a metabolism or protein synthesis machinery and rely on host cells for replication. The model organism Bacillus subtilis has served as a host strain for decades and enabled the isolation of many unique viral strains. However, many viral species representatives remained orphans as no, or only a few, related phages were ever re-isolated. The presented protocol describes how a CRISPR-Cas9 system with an artificial CRISPR-array can be set up and used to discriminate abundant and well-known B. subtilis phage from a host-based metagenome enrichment. The obtained viral suspension can be used for metagenome sequencing and isolating new viral strains.
KW - Bacillus subtilis
KW - Bacteriophage
KW - CRISPR-Cas9
KW - CutSPR
KW - gRNA
KW - Metagenome
KW - Phage
U2 - 10.1007/978-1-0716-2795-2_15
DO - 10.1007/978-1-0716-2795-2_15
M3 - Book chapter
C2 - 36306089
AN - SCOPUS:85141004031
SN - 978-1-0716-2797-6
T3 - Methods in Molecular Biology
SP - 205
EP - 212
BT - Metagenomics
A2 - Streit, Wolfgang R.
A2 - Daniel, Rolf
PB - Humana Press
ER -
ID: 333617321