CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Phages are viruses of bacteria and have been known for over a century. They do not have a metabolism or protein synthesis machinery and rely on host cells for replication. The model organism Bacillus subtilis has served as a host strain for decades and enabled the isolation of many unique viral strains. However, many viral species representatives remained orphans as no, or only a few, related phages were ever re-isolated. The presented protocol describes how a CRISPR-Cas9 system with an artificial CRISPR-array can be set up and used to discriminate abundant and well-known B. subtilis phage from a host-based metagenome enrichment. The obtained viral suspension can be used for metagenome sequencing and isolating new viral strains.
Originalsprog | Engelsk |
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Titel | Metagenomics : Methods and Protocols |
Redaktører | Wolfgang R. Streit, Rolf Daniel |
Antal sider | 8 |
Forlag | Humana Press |
Publikationsdato | 2023 |
Sider | 205-212 |
ISBN (Trykt) | 978-1-0716-2797-6 |
ISBN (Elektronisk) | 978-1-0716-2795-2 |
DOI | |
Status | Udgivet - 2023 |
Navn | Methods in Molecular Biology |
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Vol/bind | 2555 |
ISSN | 1064-3745 |
Bibliografisk note
Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
ID: 333617321